Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation

R. Elashnikov, S. Rimpelová, V. Vosmanská, Z. Kolská, K. Kolářová, O. Lyutakov, V. Švorčík

Research output: Contribution to journalArticle

Abstract

In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70% ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70% ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70% ethanol was preferable for SH-SY5Y attachment, growth and migration.

Original languageEnglish
Article number104339
JournalReactive and Functional Polymers
Volume143
DOIs
Publication statusPublished - 1 Oct 2019
Externally publishedYes

Fingerprint

Nanofibers
cellulose
Cellulose
acetate
ethanol
Ethanol
Scaffolds
Photoelectron Spectroscopy
Fourier Transform Infrared Spectroscopy
X-ray spectroscopy
X ray photoelectron spectroscopy
chemical composition
Wettability
effect
method
cellulose acetate-butyrate
Porosity
wettability
Differential Scanning Calorimetry
calorimetry

Keywords

  • Cellulose acetate butyrate
  • Nanofibers sterilization
  • Neuroblastoma cell cultivation
  • SH-SY5Y morphology
  • Tissue engineering electrospun membranes

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry
  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation. / Elashnikov, R.; Rimpelová, S.; Vosmanská, V.; Kolská, Z.; Kolářová, K.; Lyutakov, O.; Švorčík, V.

In: Reactive and Functional Polymers, Vol. 143, 104339, 01.10.2019.

Research output: Contribution to journalArticle

Elashnikov, R. ; Rimpelová, S. ; Vosmanská, V. ; Kolská, Z. ; Kolářová, K. ; Lyutakov, O. ; Švorčík, V. / Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation. In: Reactive and Functional Polymers. 2019 ; Vol. 143.
@article{b4fa3dc168e2492aaac1a48e990973c5,
title = "Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation",
abstract = "In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70{\%} ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70{\%} ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70{\%} ethanol was preferable for SH-SY5Y attachment, growth and migration.",
keywords = "Cellulose acetate butyrate, Nanofibers sterilization, Neuroblastoma cell cultivation, SH-SY5Y morphology, Tissue engineering electrospun membranes",
author = "R. Elashnikov and S. Rimpelov{\'a} and V. Vosmansk{\'a} and Z. Kolsk{\'a} and K. Kol{\'a}řov{\'a} and O. Lyutakov and V. Švorč{\'i}k",
year = "2019",
month = "10",
day = "1",
doi = "10.1016/j.reactfunctpolym.2019.104339",
language = "English",
volume = "143",
journal = "Reactive and Functional Polymers",
issn = "1381-5148",
publisher = "Elsevier",

}

TY - JOUR

T1 - Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation

AU - Elashnikov, R.

AU - Rimpelová, S.

AU - Vosmanská, V.

AU - Kolská, Z.

AU - Kolářová, K.

AU - Lyutakov, O.

AU - Švorčík, V.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70% ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70% ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70% ethanol was preferable for SH-SY5Y attachment, growth and migration.

AB - In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70% ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70% ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70% ethanol was preferable for SH-SY5Y attachment, growth and migration.

KW - Cellulose acetate butyrate

KW - Nanofibers sterilization

KW - Neuroblastoma cell cultivation

KW - SH-SY5Y morphology

KW - Tissue engineering electrospun membranes

UR - http://www.scopus.com/inward/record.url?scp=85070905231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070905231&partnerID=8YFLogxK

U2 - 10.1016/j.reactfunctpolym.2019.104339

DO - 10.1016/j.reactfunctpolym.2019.104339

M3 - Article

VL - 143

JO - Reactive and Functional Polymers

JF - Reactive and Functional Polymers

SN - 1381-5148

M1 - 104339

ER -