Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells

Ioannis G. Theodorou, Pakatip Ruenraroengsak, Andrew Gow, Stephan Schwander, Junfeng (Jim) Zhang, Kian Fan Chung, Teresa D. Tetley, Mary P. Ryan, Alexandra E. Porter

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Inhaled nanoparticles (NPs) have high-deposition rates in the alveolar region of the lung but the effects of pulmonary surfactant (PS) on nanoparticle bioreactivity are unclear. Here, the impact of PS on the stability and dissolution of ZnO nanowires (ZnONWs) was investigated, and linked with their bioreactivity in vitro with human alveolar epithelial type 1-like cells (TT1). Pre-incubation of ZnONWs with Curosurf® (a natural porcine PS) decreased their dissolution at acidic pH, through the formation of a phospholipid corona. Confocal live cell microscopy confirmed that Curosurf® lowered intracellular dissolution, thus delaying the onset of cell death compared to bare ZnONWs. Despite reducing dissolution, Curosurf® significantly increased the uptake of ZnONWs within TT1 cells, ultimately increasing their toxicity after 24 h. Although serum improved ZnONW dispersion in suspension similar to Curosurf®, it had no effect on ZnONW internalization and toxicity, indicating a unique role of PS in promoting particle uptake. In the absence of PS, ZnONW length had no effect on dissolution kinetics or degree of cellular toxicity, indicating a less important role of length in determining ZnONW bioreactivity. This work provides unique findings on the effects of PS on the stability and toxicity of ZnONWs, which could be important in the study of pulmonary toxicity and epithelial-endothelial translocation of nanoparticles in general.

    Original languageEnglish
    Pages (from-to)1351-1362
    Number of pages12
    JournalNanotoxicology
    Volume10
    Issue number9
    DOIs
    Publication statusPublished - 20 Oct 2016

    Fingerprint

    Nanowires
    Zinc Oxide
    Pulmonary Surfactants
    Zinc oxide
    Dissolution
    Surface active agents
    Epithelial Cells
    Toxicity
    Nanoparticles
    Lung
    Phospholipids
    Cell death
    Deposition rates
    Microscopy
    Suspensions
    Microscopic examination
    Cell Death
    Swine

    Keywords

    • Alveoli
    • Curosurf®
    • epithelial uptake
    • nanoparticles
    • nanotoxicity

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Toxicology

    Cite this

    Theodorou, I. G., Ruenraroengsak, P., Gow, A., Schwander, S., Zhang, J. J., Chung, K. F., ... Porter, A. E. (2016). Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells. Nanotoxicology, 10(9), 1351-1362. https://doi.org/10.1080/17435390.2016.1214762

    Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells. / Theodorou, Ioannis G.; Ruenraroengsak, Pakatip; Gow, Andrew; Schwander, Stephan; Zhang, Junfeng (Jim); Chung, Kian Fan; Tetley, Teresa D.; Ryan, Mary P.; Porter, Alexandra E.

    In: Nanotoxicology, Vol. 10, No. 9, 20.10.2016, p. 1351-1362.

    Research output: Contribution to journalArticle

    Theodorou, IG, Ruenraroengsak, P, Gow, A, Schwander, S, Zhang, JJ, Chung, KF, Tetley, TD, Ryan, MP & Porter, AE 2016, 'Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells', Nanotoxicology, vol. 10, no. 9, pp. 1351-1362. https://doi.org/10.1080/17435390.2016.1214762
    Theodorou, Ioannis G. ; Ruenraroengsak, Pakatip ; Gow, Andrew ; Schwander, Stephan ; Zhang, Junfeng (Jim) ; Chung, Kian Fan ; Tetley, Teresa D. ; Ryan, Mary P. ; Porter, Alexandra E. / Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells. In: Nanotoxicology. 2016 ; Vol. 10, No. 9. pp. 1351-1362.
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