Effect of Na+,K+,2Cl- Cotransport inhibitor bumetanide on electrical and contractile activity of smooth muscle cells in guinea pig ureter

I. V. Kovalev, M. B. Baskakov, Yana Jonovna Anfinogenova, Yu L. Borodin, A. A. Kilin, I. L. Minochenko, A. G. Popov, L. V. Kapilevich, M. A. Medvedev, S. N. Orlov

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The effect of Na+,K+,2Cl- cotransport inhibitor bumetanide on action potentials and contractions of smooth muscle cells in the ureter of guinea pigs evoked by electrical stimulation was studied by the method of double sucrose bridge. Bumetanide (10-100 μM) dose-dependently suppressed action potential and contractions of smooth muscle cells induced by 1-10 μM histamine, 10 μM mesatone, 5 mM tetraethylammonium, and 100 μM sodium nitroprusside. Our findings suggest that test substances modulate Na+,K+,2Cl- cotransport in smooth muscle cells.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Issue number2
Publication statusPublished - Aug 2003
Externally publishedYes



  • Biologically active substances
  • Bumetanide
  • Na,K,2Cl cotransport
  • Smooth muscle cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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