The effect of Na+,K+,2Cl- cotransport inhibitor bumetanide on action potentials and contractions of smooth muscle cells in the ureter of guinea pigs evoked by electrical stimulation was studied by the method of double sucrose bridge. Bumetanide (10-100 μM) dose-dependently suppressed action potential and contractions of smooth muscle cells induced by 1-10 μM histamine, 10 μM mesatone, 5 mM tetraethylammonium, and 100 μM sodium nitroprusside. Our findings suggest that test substances modulate Na+,K+,2Cl- cotransport in smooth muscle cells.
- Biologically active substances
- Na,K,2Cl cotransport
- Smooth muscle cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)