Abstract
Preliminary intravenous injection of peptide agonist of δ1-opioid receptors DPDPE (0.5 mg/kg) decreased the incidence of occlusion (10 min) and reperfusion (10 min) arrhythmias in rats. By contrast, δ2-opioid receptor agonist DSLET produced no effect on the incidence of arrhythmias provoked by coronary occlusion and reperfusion. Preliminary injection of selective δ-receptor antagonist ICI 174,864 (2.5 mg/kg) or TIPP[ψ] (0.5 mg/kg) completely abolished the antiarrhythmic effect of DPDPE. Stimulation of cardiac δ1-opioid receptors with DPDPE added to perfusion saline in concentrations of 0.1 and 0.5 mg/liter decreased the incidence of reperfusion arrhythmias. Addition of DPDPE to perfusion saline in a concentration of 0.1 mg/liter prevented reoxygenation destruction of cardiomyocytes. By contrast, no cardioprotective effect of this peptide was observed at a concentration of 0.5 mg/liter in perfusion saline or when it was injected intravenously. It is concluded that the cardioprotective and antiarrhythmic effects of DPDPE are caused by activation of cardiac δ1-opioid receptors.
| Original language | English |
|---|---|
| Pages (from-to) | 359-362 |
| Number of pages | 4 |
| Journal | Bulletin of Experimental Biology and Medicine |
| Volume | 134 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Oct 2002 |
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Keywords
- Arrhythmias
- Heart
- Ischemia
- Opioid receptors
- Reperfusion
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Effect of in vivo and in vitro stimulation of δ1-opioid receptors on myocardial resistance to arrhythmogenic action of ischemia and reperfusion. / Lasukova, T. V.; Krylatov, A. V.; Maslov, L. N.; Lishmanov, Yu B.; Gross, G. J.; Stefano, G. B.
In: Bulletin of Experimental Biology and Medicine, Vol. 134, No. 4, 01.10.2002, p. 359-362.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of in vivo and in vitro stimulation of δ1-opioid receptors on myocardial resistance to arrhythmogenic action of ischemia and reperfusion
AU - Lasukova, T. V.
AU - Krylatov, A. V.
AU - Maslov, L. N.
AU - Lishmanov, Yu B.
AU - Gross, G. J.
AU - Stefano, G. B.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Preliminary intravenous injection of peptide agonist of δ1-opioid receptors DPDPE (0.5 mg/kg) decreased the incidence of occlusion (10 min) and reperfusion (10 min) arrhythmias in rats. By contrast, δ2-opioid receptor agonist DSLET produced no effect on the incidence of arrhythmias provoked by coronary occlusion and reperfusion. Preliminary injection of selective δ-receptor antagonist ICI 174,864 (2.5 mg/kg) or TIPP[ψ] (0.5 mg/kg) completely abolished the antiarrhythmic effect of DPDPE. Stimulation of cardiac δ1-opioid receptors with DPDPE added to perfusion saline in concentrations of 0.1 and 0.5 mg/liter decreased the incidence of reperfusion arrhythmias. Addition of DPDPE to perfusion saline in a concentration of 0.1 mg/liter prevented reoxygenation destruction of cardiomyocytes. By contrast, no cardioprotective effect of this peptide was observed at a concentration of 0.5 mg/liter in perfusion saline or when it was injected intravenously. It is concluded that the cardioprotective and antiarrhythmic effects of DPDPE are caused by activation of cardiac δ1-opioid receptors.
AB - Preliminary intravenous injection of peptide agonist of δ1-opioid receptors DPDPE (0.5 mg/kg) decreased the incidence of occlusion (10 min) and reperfusion (10 min) arrhythmias in rats. By contrast, δ2-opioid receptor agonist DSLET produced no effect on the incidence of arrhythmias provoked by coronary occlusion and reperfusion. Preliminary injection of selective δ-receptor antagonist ICI 174,864 (2.5 mg/kg) or TIPP[ψ] (0.5 mg/kg) completely abolished the antiarrhythmic effect of DPDPE. Stimulation of cardiac δ1-opioid receptors with DPDPE added to perfusion saline in concentrations of 0.1 and 0.5 mg/liter decreased the incidence of reperfusion arrhythmias. Addition of DPDPE to perfusion saline in a concentration of 0.1 mg/liter prevented reoxygenation destruction of cardiomyocytes. By contrast, no cardioprotective effect of this peptide was observed at a concentration of 0.5 mg/liter in perfusion saline or when it was injected intravenously. It is concluded that the cardioprotective and antiarrhythmic effects of DPDPE are caused by activation of cardiac δ1-opioid receptors.
KW - Arrhythmias
KW - Heart
KW - Ischemia
KW - Opioid receptors
KW - Reperfusion
UR - http://www.scopus.com/inward/record.url?scp=0036820505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036820505&partnerID=8YFLogxK
U2 - 10.1023/A:1021956214398
DO - 10.1023/A:1021956214398
M3 - Article
VL - 134
SP - 359
EP - 362
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 4
ER -