Effect of in vivo and in vitro stimulation of δ1-opioid receptors on myocardial resistance to arrhythmogenic action of ischemia and reperfusion

T. V. Lasukova, A. V. Krylatov, L. N. Maslov, Yu B. Lishmanov, G. J. Gross, G. B. Stefano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Preliminary intravenous injection of peptide agonist of δ1-opioid receptors DPDPE (0.5 mg/kg) decreased the incidence of occlusion (10 min) and reperfusion (10 min) arrhythmias in rats. By contrast, δ2-opioid receptor agonist DSLET produced no effect on the incidence of arrhythmias provoked by coronary occlusion and reperfusion. Preliminary injection of selective δ-receptor antagonist ICI 174,864 (2.5 mg/kg) or TIPP[ψ] (0.5 mg/kg) completely abolished the antiarrhythmic effect of DPDPE. Stimulation of cardiac δ1-opioid receptors with DPDPE added to perfusion saline in concentrations of 0.1 and 0.5 mg/liter decreased the incidence of reperfusion arrhythmias. Addition of DPDPE to perfusion saline in a concentration of 0.1 mg/liter prevented reoxygenation destruction of cardiomyocytes. By contrast, no cardioprotective effect of this peptide was observed at a concentration of 0.5 mg/liter in perfusion saline or when it was injected intravenously. It is concluded that the cardioprotective and antiarrhythmic effects of DPDPE are caused by activation of cardiac δ1-opioid receptors.

Original languageEnglish
Pages (from-to)359-362
Number of pages4
JournalBulletin of Experimental Biology and Medicine
Volume134
Issue number4
DOIs
Publication statusPublished - 1 Oct 2002

Keywords

  • Arrhythmias
  • Heart
  • Ischemia
  • Opioid receptors
  • Reperfusion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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