Early alveolar epithelial dysfunction promotes lung inflammation in a mouse model of Hermansky-Pudlak syndrome

Elena N. Atochina-Vasserman, Sandra R. Bates, Peggy Zhang, Helen Abramova, Zhenguo Zhang, Linda Gonzales, Jian Qin Tao, Bernadette R. Gochuico, William Gahl, Chang Jiang Guo, Andrew J. Gow, Michael F. Beers, Susan Guttentag

Research output: Contribution to journalArticle

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Abstract

Rationale: The pulmonary phenotype of Hermansky-Pudlak syndrome (HPS) in adults includes foamy alveolar type 2 cells, inflammation, and lung remodeling, but there is no information about ontogeny or early disease mediators. Objectives:Toestablish theontogenyofHPSlung disease inananimal model, examine disease mediators, and relate them to patients with HPS1. Methods: Mice with mutations in both HPS1/pale ear and HPS2/AP3B1/pearl (EPPE mice) were studied longitudinally. Total lung homogenate, lung tissue sections, and bronchoalveolar lavage (BAL) were examined for phospholipid, collagen, histology, cell counts, chemokines, surfactant protein D (SP-D), and S-nitrosylated SP-D. Isolated alveolar epithelial cells were examined for expression of inflammatory mediators, and chemotaxis assays were used to assess their importance. Pulmonary function test results and BAL from patients withHPS1andnormal volunteers wereexaminedfor clinical correlation. Measurements and Main Results: EPPE mice develop increased total lung phospholipid, followed by a macrophage-predominant pulmonaryinflammation, and lung remodeling including fibrosis.BAL fluid from EPPE animals exhibited early accumulation of both SP-D and S-nitrosylated SP-D. BAL fluid from patients with HPS1 exhibited similar changes in SP-D that correlated inversely with pulmonary function. Alveolar epithelial cells demonstrated expression of both monocyte chemotactic protein (MCP)-1 and inducible nitric oxide synthase in juvenile EPPE mice. Last, BAL from EPPE mice and patients with HPS1 enhanced migration of RAW267.4 cells, which was attenuated by immunodepletion of SP-D and MCP-1. Conclusions: Inflammation is initiated from the abnormal alveolar epithelial cells in HPS, and S-nitrosylated SP-D plays a significant role in amplifying pulmonary inflammation.

Original languageEnglish
Pages (from-to)449-458
Number of pages10
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume184
Issue number4
DOIs
Publication statusPublished - 15 Aug 2011
Externally publishedYes

Fingerprint

Hermanski-Pudlak Syndrome
Pulmonary Surfactant-Associated Protein D
Pneumonia
Alveolar Epithelial Cells
Lung
Bronchoalveolar Lavage
Chemokine CCL2
Protein S
Bronchoalveolar Lavage Fluid
Phospholipids
Respiratory Function Tests
Nitric Oxide Synthase Type II
Chemotaxis
Chemokines
Cell Movement
Ear
Volunteers
Histology
Fibrosis
Collagen

Keywords

  • Hermansky-Pudlak syndrome
  • Lung remodeling
  • S-nitrosylation
  • Surfactant protein D

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Early alveolar epithelial dysfunction promotes lung inflammation in a mouse model of Hermansky-Pudlak syndrome. / Atochina-Vasserman, Elena N.; Bates, Sandra R.; Zhang, Peggy; Abramova, Helen; Zhang, Zhenguo; Gonzales, Linda; Tao, Jian Qin; Gochuico, Bernadette R.; Gahl, William; Guo, Chang Jiang; Gow, Andrew J.; Beers, Michael F.; Guttentag, Susan.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 184, No. 4, 15.08.2011, p. 449-458.

Research output: Contribution to journalArticle

Atochina-Vasserman, EN, Bates, SR, Zhang, P, Abramova, H, Zhang, Z, Gonzales, L, Tao, JQ, Gochuico, BR, Gahl, W, Guo, CJ, Gow, AJ, Beers, MF & Guttentag, S 2011, 'Early alveolar epithelial dysfunction promotes lung inflammation in a mouse model of Hermansky-Pudlak syndrome', American Journal of Respiratory and Critical Care Medicine, vol. 184, no. 4, pp. 449-458. https://doi.org/10.1164/rccm.201011-1882OC
Atochina-Vasserman, Elena N. ; Bates, Sandra R. ; Zhang, Peggy ; Abramova, Helen ; Zhang, Zhenguo ; Gonzales, Linda ; Tao, Jian Qin ; Gochuico, Bernadette R. ; Gahl, William ; Guo, Chang Jiang ; Gow, Andrew J. ; Beers, Michael F. ; Guttentag, Susan. / Early alveolar epithelial dysfunction promotes lung inflammation in a mouse model of Hermansky-Pudlak syndrome. In: American Journal of Respiratory and Critical Care Medicine. 2011 ; Vol. 184, No. 4. pp. 449-458.
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abstract = "Rationale: The pulmonary phenotype of Hermansky-Pudlak syndrome (HPS) in adults includes foamy alveolar type 2 cells, inflammation, and lung remodeling, but there is no information about ontogeny or early disease mediators. Objectives:Toestablish theontogenyofHPSlung disease inananimal model, examine disease mediators, and relate them to patients with HPS1. Methods: Mice with mutations in both HPS1/pale ear and HPS2/AP3B1/pearl (EPPE mice) were studied longitudinally. Total lung homogenate, lung tissue sections, and bronchoalveolar lavage (BAL) were examined for phospholipid, collagen, histology, cell counts, chemokines, surfactant protein D (SP-D), and S-nitrosylated SP-D. Isolated alveolar epithelial cells were examined for expression of inflammatory mediators, and chemotaxis assays were used to assess their importance. Pulmonary function test results and BAL from patients withHPS1andnormal volunteers wereexaminedfor clinical correlation. Measurements and Main Results: EPPE mice develop increased total lung phospholipid, followed by a macrophage-predominant pulmonaryinflammation, and lung remodeling including fibrosis.BAL fluid from EPPE animals exhibited early accumulation of both SP-D and S-nitrosylated SP-D. BAL fluid from patients with HPS1 exhibited similar changes in SP-D that correlated inversely with pulmonary function. Alveolar epithelial cells demonstrated expression of both monocyte chemotactic protein (MCP)-1 and inducible nitric oxide synthase in juvenile EPPE mice. Last, BAL from EPPE mice and patients with HPS1 enhanced migration of RAW267.4 cells, which was attenuated by immunodepletion of SP-D and MCP-1. Conclusions: Inflammation is initiated from the abnormal alveolar epithelial cells in HPS, and S-nitrosylated SP-D plays a significant role in amplifying pulmonary inflammation.",
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AU - Zhang, Zhenguo

AU - Gonzales, Linda

AU - Tao, Jian Qin

AU - Gochuico, Bernadette R.

AU - Gahl, William

AU - Guo, Chang Jiang

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AU - Beers, Michael F.

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