Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator

Yu M. Khodarovich, D. D. Rakhmaninova, A. M. Barishnikova, S. M. Deyev

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: A synthetic genetic chain was created—a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalMolecular Biology
Volume54
Issue number2
DOIs
Publication statusPublished - 1 Mar 2020
Externally publishedYes

Keywords

  • gene chains
  • gene therapy
  • integrator
  • oncology
  • synthetic biology
  • transactivator

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

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