Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator

Abstract

Abstract: A synthetic genetic chain was created—a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.

Original language	English
Pages (from-to)	269-273
Number of pages	5
Journal	Molecular Biology
Volume	54
Issue number	2
DOIs	https://doi.org/10.1134/S0026893320020053
Publication status	Published - 1 Mar 2020
Externally published	Yes

Keywords

gene chains
gene therapy
integrator
oncology
synthetic biology
transactivator

ASJC Scopus subject areas

Biophysics
Structural Biology

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10.1134/S0026893320020053

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@article{5bded00981ba4cea9a9ea579c608369a,

title = "Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator",

abstract = "Abstract: A synthetic genetic chain was created—a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.",

keywords = "gene chains, gene therapy, integrator, oncology, synthetic biology, transactivator",

author = "Khodarovich, {Yu M.} and Rakhmaninova, {D. D.} and Barishnikova, {A. M.} and Deyev, {S. M.}",

year = "2020",

month = mar,

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doi = "10.1134/S0026893320020053",

language = "English",

volume = "54",

pages = "269--273",

journal = "Molecular Biology",

issn = "0026-8933",

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TY - JOUR

T1 - Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator

AU - Khodarovich, Yu M.

AU - Rakhmaninova, D. D.

AU - Barishnikova, A. M.

AU - Deyev, S. M.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - Abstract: A synthetic genetic chain was created—a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.

AB - Abstract: A synthetic genetic chain was created—a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.

KW - gene chains

KW - gene therapy

KW - integrator

KW - oncology

KW - synthetic biology

KW - transactivator

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DO - 10.1134/S0026893320020053

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