Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group

A. Sjöström, V. Tolmachev, O. Lebeda, J. Koziorowski, J. Carlsson, H. Lundqvist

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68±9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume256
Issue number2
DOIs
Publication statusPublished - 1 May 2003
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Pollution
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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