TY - JOUR
T1 - Diazonium chemistry surface treatment of piezoelectric polyhydroxybutyrate scaffolds for enhanced osteoblastic cell growth
AU - Chernozem, R. V.
AU - Guselnikova, O.
AU - Surmeneva, M. A.
AU - Postnikov, P. S.
AU - Abalymov, A. A.
AU - Parakhonskiy, B. V.
AU - De Roo, N.
AU - Depla, D.
AU - Skirtach, A. G.
AU - Surmenev, R. A.
PY - 2020/9
Y1 - 2020/9
N2 - Biodegradable piezoelectric 3D polymer scaffolds attract great attention due to possibilities to mimic the functional and mechanical properties of the extracellular matrix, avoiding secondary surgery, and electrically stimulated tissue repair. However, the preservation of piezoelectric response and improvement of wettability of hydrophobic fibrous polymer scaffolds, limiting their application in tissue engineering and regenerative medicine, is a challenge. Here, a facile and mild approach is presented to improve wettability and cell spreading on the surface of piezoelectric polyhydroxybutyrate (PHB) and non-piezoelectric polycaprolactone (PCL) scaffolds. The surface of electrospun 3D fibrous scaffolds was modified by 3,4-dicarboxybenzenediazonium tosylate (ADT-(COOH)2) via photo-induced formation of aryl radicals under ultraviolet irradiation. According to scanning electron microscopy and x-ray diffraction analyses, the intrinsic structure of 3D scaffolds remains unaffected after the treatment. Meanwhile, the attachment of hydrophilic 3,4-dicaboxyphenyl groups to the surface led to an apparent decrease of the water contact angle from 127±4° to 82±1° for PCL and from 126±4° to 78±2° for PHB, i.e. resulting in the change of the scaffold's wettability from hydrophobic to hydrophilic. Furthermore, no aging of the improved wetting was observed for 21 days. The diazonium modification allows to preserve a pronounced piezoelectric response, since PHB scaffolds demonstrate a slightly reduced effective d33 from 2.5 ± 0.3 pC•N−1 to 2.1 ± 0.4 pC•N−1 and surface electric potential from 510±56 mV to 458±25 mV after surface treatment. At the same time, after C6H3-(COOH)2 grafting, osteoblastic cells were well-spread along the PCL and PHB fibrous scaffolds. Moreover, after 7 days of incubation, the cell density is increased on the surface of ADT-(COOH)2-treated fibers in comparison to that on pristine ones, while cells formed a distinct osteoblastic network.
AB - Biodegradable piezoelectric 3D polymer scaffolds attract great attention due to possibilities to mimic the functional and mechanical properties of the extracellular matrix, avoiding secondary surgery, and electrically stimulated tissue repair. However, the preservation of piezoelectric response and improvement of wettability of hydrophobic fibrous polymer scaffolds, limiting their application in tissue engineering and regenerative medicine, is a challenge. Here, a facile and mild approach is presented to improve wettability and cell spreading on the surface of piezoelectric polyhydroxybutyrate (PHB) and non-piezoelectric polycaprolactone (PCL) scaffolds. The surface of electrospun 3D fibrous scaffolds was modified by 3,4-dicarboxybenzenediazonium tosylate (ADT-(COOH)2) via photo-induced formation of aryl radicals under ultraviolet irradiation. According to scanning electron microscopy and x-ray diffraction analyses, the intrinsic structure of 3D scaffolds remains unaffected after the treatment. Meanwhile, the attachment of hydrophilic 3,4-dicaboxyphenyl groups to the surface led to an apparent decrease of the water contact angle from 127±4° to 82±1° for PCL and from 126±4° to 78±2° for PHB, i.e. resulting in the change of the scaffold's wettability from hydrophobic to hydrophilic. Furthermore, no aging of the improved wetting was observed for 21 days. The diazonium modification allows to preserve a pronounced piezoelectric response, since PHB scaffolds demonstrate a slightly reduced effective d33 from 2.5 ± 0.3 pC•N−1 to 2.1 ± 0.4 pC•N−1 and surface electric potential from 510±56 mV to 458±25 mV after surface treatment. At the same time, after C6H3-(COOH)2 grafting, osteoblastic cells were well-spread along the PCL and PHB fibrous scaffolds. Moreover, after 7 days of incubation, the cell density is increased on the surface of ADT-(COOH)2-treated fibers in comparison to that on pristine ones, while cells formed a distinct osteoblastic network.
KW - Bone tissue engineering
KW - Diazonium surface chemistry
KW - Electrospinning
KW - Piezoelectricity
KW - Scaffold
UR - http://www.scopus.com/inward/record.url?scp=85088006962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85088006962&partnerID=8YFLogxK
U2 - 10.1016/j.apmt.2020.100758
DO - 10.1016/j.apmt.2020.100758
M3 - Article
AN - SCOPUS:85088006962
VL - 20
JO - Applied Materials Today
JF - Applied Materials Today
SN - 2352-9407
M1 - 100758
ER -