Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates

Alexandra Gennadievna Pershina, Olga A. Lytkina, Pavel S. Postnikov, Marina E. Trusova, Dmitri Yu Svitich, Viktor D. Filimonov, Alexey E. Sazonov

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The present article describes the method of immobilization of biomolecules (Horseradish peroxidase) on carbon-coated iron nanoparticles using arenediazonium tosylates. As a result, a magnetically controlled complex of biomolecules and nanoparticles was created. The complex formation was confirmed by FT-IR spectroscopy. It has been shown that peroxidase attached to nanoparticles preserves its enzymatic activity.

Original languageEnglish
Title of host publicationBiochemistry and Biotechnology: Research and Development
PublisherNova Science Publishers, Inc.
Pages39-44
Number of pages6
ISBN (Print)9781621004523
Publication statusPublished - 2012

Keywords

  • Carbon-coated nanoparticles
  • Diazonium salt
  • Enzymatic activity
  • HRP
  • Immobilization
  • Surface modification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Pershina, A. G., Lytkina, O. A., Postnikov, P. S., Trusova, M. E., Svitich, D. Y., Filimonov, V. D., & Sazonov, A. E. (2012). Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. In Biochemistry and Biotechnology: Research and Development (pp. 39-44). Nova Science Publishers, Inc..