Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates

Abstract

The present article describes the method of immobilization of biomolecules (Horseradish peroxidase) on carbon-coated iron nanoparticles using arenediazonium tosylates. As a result, a magnetically controlled complex of biomolecules and nanoparticles was created. The complex formation was confirmed by FT-IR spectroscopy. It has been shown that peroxidase attached to nanoparticles preserves its enzymatic activity.

Original language	English
Title of host publication	Biochemistry and Biotechnology: Research and Development
Publisher	Nova Science Publishers, Inc.
Pages	39-44
Number of pages	6
ISBN (Print)	9781621004523
Publication status	Published - 2012

Keywords

Carbon-coated nanoparticles
Diazonium salt
Enzymatic activity
HRP
Immobilization
Surface modification

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. / Pershina, Alexandra Gennadievna; Lytkina, Olga A.; Postnikov, Pavel S.; Trusova, Marina E.; Svitich, Dmitri Yu; Filimonov, Viktor D.; Sazonov, Alexey E.

Biochemistry and Biotechnology: Research and Development. Nova Science Publishers, Inc., 2012. p. 39-44.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AU - Pershina, Alexandra Gennadievna

AU - Lytkina, Olga A.

AU - Postnikov, Pavel S.

AU - Trusova, Marina E.

AU - Svitich, Dmitri Yu

AU - Filimonov, Viktor D.

AU - Sazonov, Alexey E.

PY - 2012

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N2 - The present article describes the method of immobilization of biomolecules (Horseradish peroxidase) on carbon-coated iron nanoparticles using arenediazonium tosylates. As a result, a magnetically controlled complex of biomolecules and nanoparticles was created. The complex formation was confirmed by FT-IR spectroscopy. It has been shown that peroxidase attached to nanoparticles preserves its enzymatic activity.

AB - The present article describes the method of immobilization of biomolecules (Horseradish peroxidase) on carbon-coated iron nanoparticles using arenediazonium tosylates. As a result, a magnetically controlled complex of biomolecules and nanoparticles was created. The complex formation was confirmed by FT-IR spectroscopy. It has been shown that peroxidase attached to nanoparticles preserves its enzymatic activity.

KW - Carbon-coated nanoparticles

KW - Diazonium salt

KW - Enzymatic activity

KW - HRP

KW - Immobilization

KW - Surface modification

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