Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates

Alexandra Gennadievna Pershina, Olga A. Lytkina, Pavel S. Postnikov, Marina E. Trusova, Dmitri Yu Svitich, Viktor D. Filimonov, Alexey E. Sazonov

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The present article describes the method of immobilization of biomolecules (Horseradish peroxidase) on carbon-coated iron nanoparticles using arenediazonium tosylates. As a result, a magnetically controlled complex of biomolecules and nanoparticles was created. The complex formation was confirmed by FT-IR spectroscopy. It has been shown that peroxidase attached to nanoparticles preserves its enzymatic activity.

Original languageEnglish
Title of host publicationBiochemistry and Biotechnology: Research and Development
PublisherNova Science Publishers, Inc.
Pages39-44
Number of pages6
ISBN (Print)9781621004523
Publication statusPublished - 2012

Fingerprint

Nanoparticles
Carbon
Iron
Biomolecules
Horseradish Peroxidase
Immobilization
Peroxidase
Infrared spectroscopy
Spectrum Analysis

Keywords

  • Carbon-coated nanoparticles
  • Diazonium salt
  • Enzymatic activity
  • HRP
  • Immobilization
  • Surface modification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pershina, A. G., Lytkina, O. A., Postnikov, P. S., Trusova, M. E., Svitich, D. Y., Filimonov, V. D., & Sazonov, A. E. (2012). Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. In Biochemistry and Biotechnology: Research and Development (pp. 39-44). Nova Science Publishers, Inc..

Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. / Pershina, Alexandra Gennadievna; Lytkina, Olga A.; Postnikov, Pavel S.; Trusova, Marina E.; Svitich, Dmitri Yu; Filimonov, Viktor D.; Sazonov, Alexey E.

Biochemistry and Biotechnology: Research and Development. Nova Science Publishers, Inc., 2012. p. 39-44.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Pershina, AG, Lytkina, OA, Postnikov, PS, Trusova, ME, Svitich, DY, Filimonov, VD & Sazonov, AE 2012, Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. in Biochemistry and Biotechnology: Research and Development. Nova Science Publishers, Inc., pp. 39-44.
Pershina AG, Lytkina OA, Postnikov PS, Trusova ME, Svitich DY, Filimonov VD et al. Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. In Biochemistry and Biotechnology: Research and Development. Nova Science Publishers, Inc. 2012. p. 39-44
Pershina, Alexandra Gennadievna ; Lytkina, Olga A. ; Postnikov, Pavel S. ; Trusova, Marina E. ; Svitich, Dmitri Yu ; Filimonov, Viktor D. ; Sazonov, Alexey E. / Development of HRP-functionalized carbon-coated Iron nanoparticles using arenediazonium tosylates. Biochemistry and Biotechnology: Research and Development. Nova Science Publishers, Inc., 2012. pp. 39-44
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