Development and investigation of recombinant immunotoxin protein 4D5scFv-mCherry-PE(40)

O. N. Shilova, E. A. Souslova, A. M. Pilunov, S. M. Deyev, R. V. Petrov

Research output: Contribution to journalArticle

Abstract

Development of agents for theranostics implies combining the targeting module, the effector module, and the detection module within the same complex or recombinant protein. We have constructed, isolated, and characterized the 4D5scFv-mCherry-PE(40) protein, which exhibits fluorescent properties and specifically binds to cancer cells expressing the HER2 receptor and reduces their viability. The ability of the obtained targeted antitumor agent 4D5scFv-mCherry-PE(40) to selectively stain the HER2-positive cells and its highly selective cytotoxicity against these cells make the obtained targeted recombinant protein 4D5scFv-mCherry-PE(40) a promising theranostic agent for the diagnostics and therapy of HER2-positive human tumors.

Original languageEnglish
Pages (from-to)450-453
Number of pages4
JournalDoklady Biochemistry and Biophysics
Volume471
Issue number1
DOIs
Publication statusPublished - 1 Nov 2016
Externally publishedYes

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Immunotoxins
Recombinant proteins
Recombinant Proteins
Cells
Cytotoxicity
Antineoplastic Agents
Tumors
Coloring Agents
Neoplasms
Proteins
Theranostic Nanomedicine
Therapeutics

ASJC Scopus subject areas

  • Biophysics
  • Chemistry(all)
  • Medicine(all)
  • Biochemistry

Cite this

Development and investigation of recombinant immunotoxin protein 4D5scFv-mCherry-PE(40). / Shilova, O. N.; Souslova, E. A.; Pilunov, A. M.; Deyev, S. M.; Petrov, R. V.

In: Doklady Biochemistry and Biophysics, Vol. 471, No. 1, 01.11.2016, p. 450-453.

Research output: Contribution to journalArticle

Shilova, O. N. ; Souslova, E. A. ; Pilunov, A. M. ; Deyev, S. M. ; Petrov, R. V. / Development and investigation of recombinant immunotoxin protein 4D5scFv-mCherry-PE(40). In: Doklady Biochemistry and Biophysics. 2016 ; Vol. 471, No. 1. pp. 450-453.
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