Determination of thymol using HPLC-ED with glassy carbon paste electrode

Abstract

The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L^-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10^-7 mol L^-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.

Original language	English
Pages (from-to)	1049-1057
Number of pages	9
Journal	Chemia Analityczna
Volume	52
Issue number	6
Publication status	Published - 1 Dec 2007
Externally published	Yes

Keywords

Amperometric detection
Glassy carbon paste electrode
HPLC-ED
Thymol

ASJC Scopus subject areas

Analytical Chemistry

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title = "Determination of thymol using HPLC-ED with glassy carbon paste electrode",

abstract = "The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.",

keywords = "Amperometric detection, Glassy carbon paste electrode, HPLC-ED, Thymol",

author = "Jiri Zima and Miroslava Cienciala and Jiri Barek and Moreira, {Josino C.}",

year = "2007",

month = dec,

day = "1",

language = "English",

volume = "52",

pages = "1049--1057",

journal = "Chemia Analityczna",

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publisher = "Polskie Towarzystwo Chemiczne",

number = "6",

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T1 - Determination of thymol using HPLC-ED with glassy carbon paste electrode

AU - Zima, Jiri

AU - Cienciala, Miroslava

AU - Barek, Jiri

AU - Moreira, Josino C.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.

AB - The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.

KW - Amperometric detection

KW - Glassy carbon paste electrode

KW - HPLC-ED

KW - Thymol

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VL - 52

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JO - Chemia Analityczna

JF - Chemia Analityczna

SN - 0009-2223

IS - 6

ER -

Determination of thymol using HPLC-ED with glassy carbon paste electrode

Abstract

Keywords

ASJC Scopus subject areas

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