Abstract
The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.
| Original language | English |
|---|---|
| Pages (from-to) | 1049-1057 |
| Number of pages | 9 |
| Journal | Chemia Analityczna |
| Volume | 52 |
| Issue number | 6 |
| Publication status | Published - 1 Dec 2007 |
| Externally published | Yes |
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Keywords
- Amperometric detection
- Glassy carbon paste electrode
- HPLC-ED
- Thymol
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Determination of thymol using HPLC-ED with glassy carbon paste electrode. / Zima, Jiri; Cienciala, Miroslava; Barek, Jiri; Moreira, Josino C.
In: Chemia Analityczna, Vol. 52, No. 6, 01.12.2007, p. 1049-1057.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of thymol using HPLC-ED with glassy carbon paste electrode
AU - Zima, Jiri
AU - Cienciala, Miroslava
AU - Barek, Jiri
AU - Moreira, Josino C.
PY - 2007/12/1
Y1 - 2007/12/1
N2 - The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.
AB - The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.
KW - Amperometric detection
KW - Glassy carbon paste electrode
KW - HPLC-ED
KW - Thymol
UR - http://www.scopus.com/inward/record.url?scp=39649114545&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39649114545&partnerID=8YFLogxK
M3 - Article
VL - 52
SP - 1049
EP - 1057
JO - Chemia Analityczna
JF - Chemia Analityczna
SN - 0009-2223
IS - 6
ER -