Determination of thymol using HPLC-ED with glassy carbon paste electrode

Jiri Zima, Miroslava Cienciala, Jiri Barek, Josino C. Moreira

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12 Citations (Scopus)

Abstract

The use of glassy carbon electrodes (CPE) for the determination of tymol was studied. The amperometric detection with prior separation of tymol by HPLC is proposed. The optimum conditions have been found. The HPLC-ED limit of detection of tymol at + 1.1 V vs Ag/AgCl/3 mol L-1 KCl in the mobile phase consisting of diluted Britton-Robinson buffer pH 7-methanol 1:9 (v/v) was 2.9 × 10-7 mol L-1 which was lower than that with UV detection at 276 nm. The repeatability of the new method for the determination of thymol using HPLC with amperometric detection, expressed as the relative standard deviation, was below 2 % when the areas of the peaks were evaluated and below 5% when the peak heights were measured. The practial application of newly developed method of HPLC-ED determination was tested on samples of Septolete pastilles and thyme sirup.

Original languageEnglish
Pages (from-to)1049-1057
Number of pages9
JournalChemia Analityczna
Volume52
Issue number6
Publication statusPublished - 1 Dec 2007
Externally publishedYes

Keywords

  • Amperometric detection
  • Glassy carbon paste electrode
  • HPLC-ED
  • Thymol

ASJC Scopus subject areas

  • Analytical Chemistry

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  • Cite this

    Zima, J., Cienciala, M., Barek, J., & Moreira, J. C. (2007). Determination of thymol using HPLC-ED with glassy carbon paste electrode. Chemia Analityczna, 52(6), 1049-1057.