Determination of three Tumor Biomarkers (Homovanillic Acid, Vanillylmandelic Acid, and 5-Hydroxyindole-3-Acetic Acid) Using Flow Injection Analysis with Amperometric Detection

Anna Němečková-Makrlíková, Frank Michael Matysik, Tomáš Navrátil, Jiří Barek, Vlastimil Vyskočil

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Flow injection analysis with amperometric detection (FIA-AD) at screen-printed carbon electrodes (SPCEs) in optimum medium of Britton-Robinson buffer (0.04 mol ⋅ L −1 , pH 2.0) was used for the determination of three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)). Dependences of the peak current on the concentration of biomarkers were linear in the whole tested concentration range from 0.05 to 100 μmol ⋅ L −1 , with limits of detection (LODs) of 0.065 μmol ⋅ L −1 for HVA, 0.053 μmol ⋅ L −1 for VMA, and 0.033 μmol ⋅ L −1 for 5-HIAA (calculated from peak heights), and 0.024 μmol ⋅ L −1 for HVA, 0.020 μmol ⋅ L −1 for VMA, and 0.012 μmol ⋅ L −1 for 5-HIAA (calculated from peak areas), respectively.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
Issue number2
Publication statusPublished - 1 Feb 2019
Externally publishedYes



  • 5-Hydroxyindole-3-acetic acid
  • Amperometric detection
  • Flow injection analysis
  • Homovanillic acid
  • Screen-printed carbon electrodes
  • Vanillylmandelic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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