Determination of chloramphenicol by differential pulse voltammetry at carbon paste electrodes - The use of sodium sulfite for removal of oxygen from electrode surface

Ferenc T. Pastor, Hana Dejmková, Jiří Zima, Jiří Barek

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in the 10-6 and 10-5 M chloramphenicol concentration range. Limit of determination was 5 × 10-7 mol/l.

Original languageEnglish
Pages (from-to)383-397
Number of pages15
JournalCollection of Czechoslovak Chemical Communications
Volume76
Issue number5
DOIs
Publication statusPublished - 10 May 2011
Externally publishedYes

Fingerprint

Chloramphenicol
Voltammetry
Ointments
Carbon
Oxygen
Electrodes
Tritolyl Phosphates
Glassy carbon
Buffers
Calibration
Mineral Oil
Graphite
Powders
sodium sulfite

Keywords

  • Carbon paste electrodes
  • Chloramphenicol
  • Differential pulse voltammetry
  • Oxygen peak
  • Oxygen removal
  • Sodium sulfite

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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abstract = "The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in the 10-6 and 10-5 M chloramphenicol concentration range. Limit of determination was 5 × 10-7 mol/l.",
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AU - Pastor, Ferenc T.

AU - Dejmková, Hana

AU - Zima, Jiří

AU - Barek, Jiří

PY - 2011/5/10

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N2 - The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in the 10-6 and 10-5 M chloramphenicol concentration range. Limit of determination was 5 × 10-7 mol/l.

AB - The possibility of determination of chloramphenicol by differential pulse voltammetry at four different carbon paste electrodes, in the full pH range (2-12) of Britton-Robinson (BR) buffer was investigated. Electrodes were prepared by mixing spectroscopic graphite powder or glassy carbon microbeads with mineral oil (Nujol) or tricresyl phosphate. Under optimal conditions (BR buffer pH 12, the electrode prepared from glassy carbon microbeads and tricresyl phosphate), linear calibration graph was obtained only in 10-5 M chloramphenicol concentration range. Determination of lower concentrations of chloramphenicol was complicated by irreproducible peak of oxygen from the carbon paste which overlapped with peak of chloramphenicol. Addition of sodium sulfite removed the oxygen peak without influence on the peak of chloramphenicol. Under optimal conditions (electrode paste made from glassy carbon microbeads, BR buffer pH 10 and 0.5 M sodium sulfite), straight calibration line was obtained in the 10-6 and 10-5 M chloramphenicol concentration range. Limit of determination was 5 × 10-7 mol/l.

KW - Carbon paste electrodes

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KW - Oxygen peak

KW - Oxygen removal

KW - Sodium sulfite

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