Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode

Hana Dejmkova, Veronika Vokalova, Jiri Zima, Jiri Barek

Research output: Contribution to journalArticle

10 Citations (Scopus)


A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.

Original languageEnglish
Pages (from-to)662-666
Number of pages5
Issue number3
Publication statusPublished - 1 Mar 2011
Externally publishedYes



  • Amperometric detection
  • Carbon paste electrode
  • FIA
  • HPLC
  • Pharmaceuticals

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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