Abstract
A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.
| Original language | English |
|---|---|
| Pages (from-to) | 662-666 |
| Number of pages | 5 |
| Journal | Electroanalysis |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Mar 2011 |
| Externally published | Yes |
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Keywords
- Amperometric detection
- Carbon paste electrode
- FIA
- HPLC
- Pharmaceuticals
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode. / Dejmkova, Hana; Vokalova, Veronika; Zima, Jiri; Barek, Jiri.
In: Electroanalysis, Vol. 23, No. 3, 01.03.2011, p. 662-666.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode
AU - Dejmkova, Hana
AU - Vokalova, Veronika
AU - Zima, Jiri
AU - Barek, Jiri
PY - 2011/3/1
Y1 - 2011/3/1
N2 - A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.
AB - A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.
KW - Amperometric detection
KW - Carbon paste electrode
KW - FIA
KW - HPLC
KW - Pharmaceuticals
UR - http://www.scopus.com/inward/record.url?scp=79954625849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79954625849&partnerID=8YFLogxK
U2 - 10.1002/elan.201000646
DO - 10.1002/elan.201000646
M3 - Article
VL - 23
SP - 662
EP - 666
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 3
ER -