Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode

Abstract

A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin^-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin^-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.

Original language	English
Pages (from-to)	662-666
Number of pages	5
Journal	Electroanalysis
Volume	23
Issue number	3
DOIs	https://doi.org/10.1002/elan.201000646
Publication status	Published - 1 Mar 2011
Externally published	Yes

Keywords

Amperometric detection
Carbon paste electrode
FIA
HPLC
Pharmaceuticals

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.201000646

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title = "Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode",

abstract = "A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.",

keywords = "Amperometric detection, Carbon paste electrode, FIA, HPLC, Pharmaceuticals",

author = "Hana Dejmkova and Veronika Vokalova and Jiri Zima and Jiri Barek",

year = "2011",

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doi = "10.1002/elan.201000646",

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T1 - Determination of Benzocaine Using HPLC and FIA with Amperometric Detection on a Carbon Paste Electrode

AU - Dejmkova, Hana

AU - Vokalova, Veronika

AU - Zima, Jiri

AU - Barek, Jiri

PY - 2011/3/1

Y1 - 2011/3/1

N2 - A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.

AB - A new method for benzocaine determination employing FIA and HPLC with electrochemical detection on a carbon paste electrode was developed. The optimum conditions for the determination were found. Carrier solution for FIA consisted of B-R buffer pH4 (80% methanol, v/v) and used flow rate was 1.0mLmin-1. Mobile phase for HPLC consisted of B-R buffer pH4 (75% methanol, v/v) with flow rate 0.4mLmin-1. Working potential of +1.2V was employed. Practical applicability of the methods was tested on the determination of benzocaine in selected pharmaceuticals. The results were in agreement with results obtained using spectrophotometric detection and with one exception also with the content declared by the manufacturer.

KW - Amperometric detection

KW - Carbon paste electrode

KW - FIA

KW - HPLC

KW - Pharmaceuticals

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