Determination of 4-aminobiphenyl and 4-nitrobiphenyl by HPLC with electrochemical, UV spectrophotometric, and fluorescent detection

Vít Prachal, Jana Krejčová, Vlastimil Vyskočil, Karolina Pecková, Jan Fischer, Jíří Zima, Jiří Barek

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

HPLC systems with electrochemical, UV spectrophotometric, and fluorescent detectors were used to determine genotoxic derivatives of biphenyl - 4-aminobiphenyl (4-ABP) and 4-nitrobiphenyl (4-NBP). As 4-NBP cannot be determined by electrochemical and fluorescent detector directly, reduction of 4-NBP to 4-ABP (making use of a titanium(III)-based reducing agent) was investigated and optimized. UV detection was found to be the least sensitive and fluorescent detection the most sensitive one. Limits of detection (LDs) for 4-ABP in the presence of a reducing agent were 6.0 × 10-6 mol L-1, 2.0 × 10-6 mol L-1, and 8.0 × 10-8 mol L-1for UV, electrochemical, and fluorescent detection, respectively. For the determination of 4-NBP after its reduction to 4-ABP, the obtained LDs were 8.0 × 10-6 mol L-1 (UV), 4.0 × 10-6 mol L-1 (electrochemical), and 2.0 × 10-7 mol L-1 (fluorescent detection). Moreover, it wasalso possible to determine 4-NBP directly using HPLC with UV detection, where the LD was 4.0 × 10-7 molL-1.

Original languageEnglish
Pages (from-to)2524-2535
Number of pages12
JournalInternational Journal of Electrochemical Science
Volume8
Issue number2
Publication statusPublished - 1 Feb 2013
Externally publishedYes

Fingerprint

Reducing agents
Detectors
Reducing Agents
Titanium
Derivatives
4-biphenylamine
4-nitrobiphenyl

Keywords

  • Electrochemical detection
  • Fluorescent detection
  • Genotoxic derivatives of biphenyl
  • High performance liquid chromatography
  • Platinum tubular detector
  • UV spectrophotometric detection

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Determination of 4-aminobiphenyl and 4-nitrobiphenyl by HPLC with electrochemical, UV spectrophotometric, and fluorescent detection. / Prachal, Vít; Krejčová, Jana; Vyskočil, Vlastimil; Pecková, Karolina; Fischer, Jan; Zima, Jíří; Barek, Jiří.

In: International Journal of Electrochemical Science, Vol. 8, No. 2, 01.02.2013, p. 2524-2535.

Research output: Contribution to journalArticle

Prachal, Vít ; Krejčová, Jana ; Vyskočil, Vlastimil ; Pecková, Karolina ; Fischer, Jan ; Zima, Jíří ; Barek, Jiří. / Determination of 4-aminobiphenyl and 4-nitrobiphenyl by HPLC with electrochemical, UV spectrophotometric, and fluorescent detection. In: International Journal of Electrochemical Science. 2013 ; Vol. 8, No. 2. pp. 2524-2535.
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