Determination of 4-aminobiphenyl and 4-nitrobiphenyl by HPLC with electrochemical, UV spectrophotometric, and fluorescent detection

Vít Prachal, Jana Krejčová, Vlastimil Vyskočil, Karolina Pecková, Jan Fischer, Jíří Zima, Jiří Barek

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4 Citations (Scopus)


HPLC systems with electrochemical, UV spectrophotometric, and fluorescent detectors were used to determine genotoxic derivatives of biphenyl - 4-aminobiphenyl (4-ABP) and 4-nitrobiphenyl (4-NBP). As 4-NBP cannot be determined by electrochemical and fluorescent detector directly, reduction of 4-NBP to 4-ABP (making use of a titanium(III)-based reducing agent) was investigated and optimized. UV detection was found to be the least sensitive and fluorescent detection the most sensitive one. Limits of detection (LDs) for 4-ABP in the presence of a reducing agent were 6.0 × 10-6 mol L-1, 2.0 × 10-6 mol L-1, and 8.0 × 10-8 mol L-1for UV, electrochemical, and fluorescent detection, respectively. For the determination of 4-NBP after its reduction to 4-ABP, the obtained LDs were 8.0 × 10-6 mol L-1 (UV), 4.0 × 10-6 mol L-1 (electrochemical), and 2.0 × 10-7 mol L-1 (fluorescent detection). Moreover, it wasalso possible to determine 4-NBP directly using HPLC with UV detection, where the LD was 4.0 × 10-7 molL-1.

Original languageEnglish
Pages (from-to)2524-2535
Number of pages12
JournalInternational Journal of Electrochemical Science
Issue number2
Publication statusPublished - 1 Feb 2013
Externally publishedYes



  • Electrochemical detection
  • Fluorescent detection
  • Genotoxic derivatives of biphenyl
  • High performance liquid chromatography
  • Platinum tubular detector
  • UV spectrophotometric detection

ASJC Scopus subject areas

  • Electrochemistry

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