Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection

J. Barek, V. Bencko, J. Cvačka, V. Mejstřík, A. Slámová, I. Švagrová, J. Zima

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Abstract

Optimum conditions are described for HPLC determination of 1-hydroxypyrene with electrochemical detection (ED) giving the detection limit ∼ 1.10-8 mol.l-1. This detection limit was compared with that for UV spectrophotometric detection (∼ 1.10-7 mol.l-1) and fluorimetric detection (∼ 5.10-10 mol.l-1). It was demonstrated that HPLC-ED can be used for the determination of 1-hydroxypyrene in urine down to 1.10-7 mol.l-1 and thus for biological monitoring of human exposure to polycyclic aromatic hydrocarbons.

Original languageEnglish
Pages (from-to)875-876
Number of pages2
JournalChemicke Listy
Volume91
Issue number10
Publication statusPublished - 1 Dec 1997
Externally publishedYes

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ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Barek, J., Bencko, V., Cvačka, J., Mejstřík, V., Slámová, A., Švagrová, I., & Zima, J. (1997). Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection. Chemicke Listy, 91(10), 875-876.