Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection

Abstract

Optimum conditions are described for HPLC determination of 1-hydroxypyrene with electrochemical detection (ED) giving the detection limit ∼ 1.10^-8 mol.l^-1. This detection limit was compared with that for UV spectrophotometric detection (∼ 1.10^-7 mol.l^-1) and fluorimetric detection (∼ 5.10^-10 mol.l^-1). It was demonstrated that HPLC-ED can be used for the determination of 1-hydroxypyrene in urine down to 1.10^-7 mol.l^-1 and thus for biological monitoring of human exposure to polycyclic aromatic hydrocarbons.

Original language	English
Pages (from-to)	875-876
Number of pages	2
Journal	Chemicke Listy
Volume	91
Issue number	10
Publication status	Published - 1 Dec 1997
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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Barek, J., Bencko, V., Cvačka, J., Mejstřík, V., Slámová, A., Švagrová, I., & Zima, J. (1997). Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection. Chemicke Listy, 91(10), 875-876.

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title = "Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection",

abstract = "Optimum conditions are described for HPLC determination of 1-hydroxypyrene with electrochemical detection (ED) giving the detection limit ∼ 1.10-8 mol.l-1. This detection limit was compared with that for UV spectrophotometric detection (∼ 1.10-7 mol.l-1) and fluorimetric detection (∼ 5.10-10 mol.l-1). It was demonstrated that HPLC-ED can be used for the determination of 1-hydroxypyrene in urine down to 1.10-7 mol.l-1 and thus for biological monitoring of human exposure to polycyclic aromatic hydrocarbons.",

author = "J. Barek and V. Bencko and J. Cva{\v c}ka and V. Mejst{\v r}{\'i}k and A. Sl{\'a}mov{\'a} and I. {\v S}vagrov{\'a} and J. Zima",

year = "1997",

month = dec,

day = "1",

language = "English",

volume = "91",

pages = "875--876",

journal = "Chemicke Listy",

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T1 - Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection

AU - Barek, J.

AU - Bencko, V.

AU - Cvačka, J.

AU - Mejstřík, V.

AU - Slámová, A.

AU - Švagrová, I.

AU - Zima, J.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Optimum conditions are described for HPLC determination of 1-hydroxypyrene with electrochemical detection (ED) giving the detection limit ∼ 1.10-8 mol.l-1. This detection limit was compared with that for UV spectrophotometric detection (∼ 1.10-7 mol.l-1) and fluorimetric detection (∼ 5.10-10 mol.l-1). It was demonstrated that HPLC-ED can be used for the determination of 1-hydroxypyrene in urine down to 1.10-7 mol.l-1 and thus for biological monitoring of human exposure to polycyclic aromatic hydrocarbons.

AB - Optimum conditions are described for HPLC determination of 1-hydroxypyrene with electrochemical detection (ED) giving the detection limit ∼ 1.10-8 mol.l-1. This detection limit was compared with that for UV spectrophotometric detection (∼ 1.10-7 mol.l-1) and fluorimetric detection (∼ 5.10-10 mol.l-1). It was demonstrated that HPLC-ED can be used for the determination of 1-hydroxypyrene in urine down to 1.10-7 mol.l-1 and thus for biological monitoring of human exposure to polycyclic aromatic hydrocarbons.

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