Denaturation-resistant bifunctional colloidal superstructures assembled via the proteinaceous barnase-barstar interface

Ulkar F. Aghayeva, Maxim P. Nikitin, Sergey V. Lukash, Sergey M. Deyev

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To date, a number of biomolecule-mediated nanoparticle self-assembly systems have been developed that are amenable to controllable disassembly under relatively gentle conditions. However, for some applications such as design of self-assembled multifunctional theragnostic agents, high stability of the assembled structures can be of primary importance. Here, we report extraordinarily high durability of protein-assisted nanoparticle self-assembly systems yielding bifunctional colloidal superstructures resistant to extreme denaturing conditions intolerable for most proteins (e.g., high concentrations of chaotropic agents, high temperature). Among the tested systems (barnase-barstar (BBS), streptavidin-biotin, antibody-antigen, and protein A-immunoglobulin), the BBS is notable due to the combination of its high resistance to severe chemical perturbation and unique advantages offered by genetic engineering of this entirely protein-based system. Comparison of the self-assembly systems shows that whereas in all cases the preassembled structures proved essentially resistant to extreme conditions, the ability of the complementary biomolecular pairs to mediate assembly of the initial biomolecule-particle conjugates differs substantially in these conditions.

Original languageEnglish
Pages (from-to)950-961
Number of pages12
JournalACS Nano
Volume7
Issue number2
DOIs
Publication statusPublished - 26 Feb 2013
Externally publishedYes

Keywords

  • colloidal stability
  • denaturing agents
  • disassembly in extreme conditions
  • multifunctional hybrid heterostructures
  • protein-assisted self-assembly of nanoparticles

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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