Delayed clearance of Pneumocystis carinii infection, increased inflammation, and altered nitric oxide metabolism in lungs of surfactant protein-D knockout mice

Elena Nikolaevna Atochina, Andrew J. Gow, James M. Beck, Angela Haczku, Adam Inch, Helchem Kadire, Yaniv Tomer, Christine Davis, Angela M. Preston, Francis Poulain, Samuel Hawgood, Michael F. Beers

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Surfactant protein-D (SP-D), a member of the "collectin" family, has been shown to play a role in innate immunity through modulation of inflammation and clearance of organisms. The role of SP-D in host defense against Pneumocystis carinii pneumonia was assessed using SP-D knockout (KO) mice. When inoculated with P. carinii, both wild-type (wt) and SP-D KO mice required CD4 cell depletion to develop infection. In CD4 cell-depleted models, 2 weeks after infection with P. carinii, SP-D KO mice developed increased intensity of infection, compared with wt mice, despite higher lung-inflammation scores and increased amounts of alveolar inflammatory cells. The increased inflammation seen in SP-D KO mice was accompanied by increases in lung weight, expression of inducible nitric oxide (NO) synthase, total NO levels, and 3-nitrotyrosine levels in lung tissue. These results indicate that SP-D plays a role in host defense against P. carinii in vivo by modulating clearance of organisms, lung inflammation, and metabolism of NO.

Original languageEnglish
Pages (from-to)1528-1539
Number of pages12
JournalJournal of Infectious Diseases
Volume189
Issue number8
DOIs
Publication statusPublished - 15 Apr 2004
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactant-Associated Protein D
Pneumocystis Infections
Knockout Mice
Nitric Oxide
Inflammation
Pneumocystis carinii
Pneumonia
Collectins
Alveolar Epithelial Cells
Lung
Pneumocystis Pneumonia
Nitric Oxide Synthase Type II
Infection
Innate Immunity
Weights and Measures

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Delayed clearance of Pneumocystis carinii infection, increased inflammation, and altered nitric oxide metabolism in lungs of surfactant protein-D knockout mice. / Atochina, Elena Nikolaevna; Gow, Andrew J.; Beck, James M.; Haczku, Angela; Inch, Adam; Kadire, Helchem; Tomer, Yaniv; Davis, Christine; Preston, Angela M.; Poulain, Francis; Hawgood, Samuel; Beers, Michael F.

In: Journal of Infectious Diseases, Vol. 189, No. 8, 15.04.2004, p. 1528-1539.

Research output: Contribution to journalArticle

Atochina, EN, Gow, AJ, Beck, JM, Haczku, A, Inch, A, Kadire, H, Tomer, Y, Davis, C, Preston, AM, Poulain, F, Hawgood, S & Beers, MF 2004, 'Delayed clearance of Pneumocystis carinii infection, increased inflammation, and altered nitric oxide metabolism in lungs of surfactant protein-D knockout mice', Journal of Infectious Diseases, vol. 189, no. 8, pp. 1528-1539. https://doi.org/10.1086/383130
Atochina, Elena Nikolaevna ; Gow, Andrew J. ; Beck, James M. ; Haczku, Angela ; Inch, Adam ; Kadire, Helchem ; Tomer, Yaniv ; Davis, Christine ; Preston, Angela M. ; Poulain, Francis ; Hawgood, Samuel ; Beers, Michael F. / Delayed clearance of Pneumocystis carinii infection, increased inflammation, and altered nitric oxide metabolism in lungs of surfactant protein-D knockout mice. In: Journal of Infectious Diseases. 2004 ; Vol. 189, No. 8. pp. 1528-1539.
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