Decreased bacterial colonization of additively manufactured Ti6Al4V metallic scaffolds with immobilized silver and calcium phosphate nanoparticles

Maria Surmeneva, Ales Lapanje, Ekaterina Chudinova, Anna Ivanova, Andrey Koptyug, Kateryna Loza, Oleg Prymak, Matthias Epple, Franka Ennen-Roth, Mathias Ulbricht, Tomaž Rijavec, Roman Surmenev

Research output: Contribution to journalArticle

Abstract

The design of an ideal bone graft substitute has been a long-standing effort, and a number of strategies have been developed to improve bone regeneration. Electron beam melting (EBM) is an additive manufacturing method allowing for the production of porous implants with highly defined external dimensions and internal architectures. The increasing surface area of the implant may also increase the abilities of pathogenic microorganisms to adhere to the surfaces and form a biofilm, which may result in serious complications. The aim of this study was to explore the modifications of Ti6Al4V alloy scaffolds to reduce the abilities of bacteria to attach to the EBM-manufactured implant surface. The layers composed of silver (Ag), calcium phosphate (CaP) nanoparticles (NPs) and combinations of both were formed on the EBM-fabricated metallic scaffolds by electrophoretic deposition in order to provide them with antimicrobial properties. The assay of bacterial colonization on the surface was performed with the exposure of scaffold surfaces to Staphylococcus aureus cells for up to 17 h. Principal component analysis (PCA) was used to assess the relationships between different surface features of the studied samples and bacterial adhesion. The results indicate that by modifying the implant surface with appropriate nanostructures that change the hydrophobicity and the surface roughness at the nano scale, physical cues are provided that disrupt bacterial adhesion. Our results clearly show that AgNPs at a concentration of approximately 0.02 mg/сm 2 that were deposited together with CaPNPs covered by positively charge polyethylenimine (PEI) on the surface of EBM-sintered Ti6Al4V scaffolds hindered bacterial growth, as the total number of attached cells (NAC) of S. aureus remained at the same level during the 17 h of exposure, which indicates bacteriostatic activity.

Original languageEnglish
Pages (from-to)822-829
Number of pages8
JournalApplied Surface Science
Volume480
DOIs
Publication statusPublished - 30 Jun 2019

Fingerprint

Calcium phosphate
Silver
Scaffolds
Electron beam melting
Nanoparticles
Bone
3D printers
Adhesion
Polyethyleneimine
titanium alloy (TiAl6V4)
calcium phosphate
Biofilms
Hydrophobicity
Grafts
Microorganisms
Principal component analysis
Assays
Nanostructures
Bacteria
Surface roughness

Keywords

  • Additive manufacturing
  • Antimicrobial assay
  • Bacteriostatic activity
  • Electron beam melting
  • Electrophoretic deposition
  • Nanoparticles

ASJC Scopus subject areas

  • Surfaces, Coatings and Films

Cite this

Decreased bacterial colonization of additively manufactured Ti6Al4V metallic scaffolds with immobilized silver and calcium phosphate nanoparticles. / Surmeneva, Maria; Lapanje, Ales; Chudinova, Ekaterina; Ivanova, Anna; Koptyug, Andrey; Loza, Kateryna; Prymak, Oleg; Epple, Matthias; Ennen-Roth, Franka; Ulbricht, Mathias; Rijavec, Tomaž; Surmenev, Roman.

In: Applied Surface Science, Vol. 480, 30.06.2019, p. 822-829.

Research output: Contribution to journalArticle

Surmeneva, Maria ; Lapanje, Ales ; Chudinova, Ekaterina ; Ivanova, Anna ; Koptyug, Andrey ; Loza, Kateryna ; Prymak, Oleg ; Epple, Matthias ; Ennen-Roth, Franka ; Ulbricht, Mathias ; Rijavec, Tomaž ; Surmenev, Roman. / Decreased bacterial colonization of additively manufactured Ti6Al4V metallic scaffolds with immobilized silver and calcium phosphate nanoparticles. In: Applied Surface Science. 2019 ; Vol. 480. pp. 822-829.
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AU - Ivanova, Anna

AU - Koptyug, Andrey

AU - Loza, Kateryna

AU - Prymak, Oleg

AU - Epple, Matthias

AU - Ennen-Roth, Franka

AU - Ulbricht, Mathias

AU - Rijavec, Tomaž

AU - Surmenev, Roman

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