Abstract
Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).
| Original language | English |
|---|---|
| Pages (from-to) | 351-354 |
| Number of pages | 4 |
| Journal | Materials Letters |
| Volume | 158 |
| DOIs | |
| Publication status | Published - 22 Jun 2015 |
| Externally published | Yes |
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Keywords
- Cytotoxicity
- Metabolic activity
- Metal nanoparticles
- Sputtering
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering
- Mechanics of Materials
Cite this
Cytotoxicity of noble metal nanoparticles sputtered into glycerol. / Staszek, Marek; Siegel, Jakub; Rimpelová, Silvie; Lyutakov, Oleksiy; Švorčík, Václav.
In: Materials Letters, Vol. 158, 22.06.2015, p. 351-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytotoxicity of noble metal nanoparticles sputtered into glycerol
AU - Staszek, Marek
AU - Siegel, Jakub
AU - Rimpelová, Silvie
AU - Lyutakov, Oleksiy
AU - Švorčík, Václav
PY - 2015/6/22
Y1 - 2015/6/22
N2 - Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).
AB - Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).
KW - Cytotoxicity
KW - Metabolic activity
KW - Metal nanoparticles
KW - Sputtering
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UR - http://www.scopus.com/inward/citedby.url?scp=84934893023&partnerID=8YFLogxK
U2 - 10.1016/j.matlet.2015.06.021
DO - 10.1016/j.matlet.2015.06.021
M3 - Article
VL - 158
SP - 351
EP - 354
JO - Materials Letters
JF - Materials Letters
SN - 0167-577X
ER -