Cytotoxicity of noble metal nanoparticles sputtered into glycerol

Abstract

Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).

Original language	English
Pages (from-to)	351-354
Number of pages	4
Journal	Materials Letters
Volume	158
DOIs	https://doi.org/10.1016/j.matlet.2015.06.021
Publication status	Published - 22 Jun 2015
Externally published	Yes

Keywords

Cytotoxicity
Metabolic activity
Metal nanoparticles
Sputtering

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanical Engineering
Mechanics of Materials

Access to Document

10.1016/j.matlet.2015.06.021

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Staszek, M., Siegel, J., Rimpelová, S., Lyutakov, O., & Švorčík, V. (2015). Cytotoxicity of noble metal nanoparticles sputtered into glycerol. Materials Letters, 158, 351-354. https://doi.org/10.1016/j.matlet.2015.06.021

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title = "Cytotoxicity of noble metal nanoparticles sputtered into glycerol",

abstract = "Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).",

keywords = "Cytotoxicity, Metabolic activity, Metal nanoparticles, Sputtering",

author = "Marek Staszek and Jakub Siegel and Silvie Rimpelov{\'a} and Oleksiy Lyutakov and V{\'a}clav {\v S}vor{\v c}{\'i}k",

year = "2015",

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doi = "10.1016/j.matlet.2015.06.021",

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AU - Staszek, Marek

AU - Siegel, Jakub

AU - Rimpelová, Silvie

AU - Lyutakov, Oleksiy

AU - Švorčík, Václav

PY - 2015/6/22

Y1 - 2015/6/22

N2 - Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).

AB - Nowadays, nanomaterials belong among one of the most studies materials. Even despite the immense advantages of nanostructured materials, some studies indicate that these materials can also harmfully affect both human being and the environment. Therefore, in this study, we have focused on nanoparticle (NP) cytotoxicity in vitro. To study NPs intrinsic biological properties, we employed cathodic sputtering into glycerol for their synthesis, thus eliminating the use of toxic solvents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated NPs of the following sizes: 6.1±1.0 nm for gold, 4.2±0.9 nm for silver, 2.5±0.6 nm for palladium, and 1.8±0.4 nm for platinum NPs. The cytotoxic activity of these NPs was determined by WST-1 assay using six model cell lines: human cells from hepatocarcinoma (Hep G2), human keratinocytes (HaCaT), mouse macrophages (RAW 264.7), mouse embryonic fibroblasts (L929 and NIH 3T3), and cells from Chinese hamster ovary (CHO-K1).

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KW - Metabolic activity

KW - Metal nanoparticles

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