TY - JOUR
T1 - Cytotoxic effects of upconversion nanoparticles in primary hippocampal cultures
AU - Vedunova, Maria V.
AU - Mishchenko, Tatiana A.
AU - Mitroshina, Elena V.
AU - Ponomareva, Natalia V.
AU - Yudintsev, Andrei V.
AU - Generalova, Alla N.
AU - Deyev, Sergey M.
AU - Mukhina, Irina V.
AU - Semyanov, Alexey V.
AU - Zvyagin, Andrei V.
PY - 2016
Y1 - 2016
N2 - The widespread use of nanomaterials causes public concerns associated with their potential toxicological hazards. New-generation nanomaterials - upconversion nanoparticles (UCNPs) - hold promise for theranostics applications due to their unique optical properties, enabling imaging at the sub-centimetre depth in live biological tissue. In brain tissue, nanoparticle-aided optical imaging and treatment are deemed desirable. To this aim, we carried out cytotoxicity studies of UCNPs in primary hippocampal cultures. The most common core/shell UCNPs (NaYF4:Yb3+:Tm3+/NaYF4) were synthesized using a solvothermal method and hydrophilized with amphiphilic polymaleic anhydride octadecene (PMAO); polyethyleneimine (PEI). Bare UCNPs were produced by using tetramethyl ammonium hydroxide (TMAH). PMAO-, PEI- and TMAH-UCNPs (0.8 mg mL-1) were incubated for 72 hours with primary hippocampal culture and exhibited noticeable cytotoxicity. Our studies showed profound morphological modification of all treated cells with the maximum and minimum uptake observed in PMAO- and TMAH-UCNP-treated cells, respectively. The spontaneous calcium activity in cells treated with TMAH-UCNP, PMAO-UCNP dropped to (17 ± 3)%, (6 ± 3)% of its original level and was completely inhibited in the PEI-UCNP-treated cultures. This study demonstrated that bare and polymer surface-coated upconversion nanoparticles are toxic to dissociated hippocampal cells, evident through aberrant morphological changes, deviant variations of Ca2+ activity, and cell death.
AB - The widespread use of nanomaterials causes public concerns associated with their potential toxicological hazards. New-generation nanomaterials - upconversion nanoparticles (UCNPs) - hold promise for theranostics applications due to their unique optical properties, enabling imaging at the sub-centimetre depth in live biological tissue. In brain tissue, nanoparticle-aided optical imaging and treatment are deemed desirable. To this aim, we carried out cytotoxicity studies of UCNPs in primary hippocampal cultures. The most common core/shell UCNPs (NaYF4:Yb3+:Tm3+/NaYF4) were synthesized using a solvothermal method and hydrophilized with amphiphilic polymaleic anhydride octadecene (PMAO); polyethyleneimine (PEI). Bare UCNPs were produced by using tetramethyl ammonium hydroxide (TMAH). PMAO-, PEI- and TMAH-UCNPs (0.8 mg mL-1) were incubated for 72 hours with primary hippocampal culture and exhibited noticeable cytotoxicity. Our studies showed profound morphological modification of all treated cells with the maximum and minimum uptake observed in PMAO- and TMAH-UCNP-treated cells, respectively. The spontaneous calcium activity in cells treated with TMAH-UCNP, PMAO-UCNP dropped to (17 ± 3)%, (6 ± 3)% of its original level and was completely inhibited in the PEI-UCNP-treated cultures. This study demonstrated that bare and polymer surface-coated upconversion nanoparticles are toxic to dissociated hippocampal cells, evident through aberrant morphological changes, deviant variations of Ca2+ activity, and cell death.
UR - http://www.scopus.com/inward/record.url?scp=84964433644&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964433644&partnerID=8YFLogxK
U2 - 10.1039/c6ra01272h
DO - 10.1039/c6ra01272h
M3 - Article
AN - SCOPUS:84964433644
VL - 6
SP - 33656
EP - 33665
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 40
ER -