Controlled release of C-type natriuretic peptide by microencapsulation dampens proinflammatory effects induced by IL-1β in cartilage explants

Nick J. Peake, Anton M. Pavlov, Alveena D'Souza, Belinda Pingguan-Murphy, Gleb B. Sukhorukov, Adrian J. Hobbs, Tina T. Chowdhury

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

C-type natriuretic peptide (CNP) exhibits potent anti-inflammatory effects in chondrocytes that have the potential to repair cartilage damage observed in osteoarthritis (OA). However, treatments for OA have been challenging due to poor targeting and delivery of therapeutics. The present study fabricated polyelectrolyte microcapsules loaded with CNP and examined whether the layer-by-layer (LbL) approach could have protective effects in cartilage explants treated with the pro-inflammatory cytokine, interleukin-1β (IL-1β). SEM showed uniform, 2 to 3 μm spherical microcapsules with morphological characteristic similar to templates loaded with or without CNP. The protein was localized around the external surface of the microcapsules with encapsulation efficiencies >82.9%. CNP release profiles were broadly similar following 9 days of culture. The presence of CNP microcapsules did not significantly affect cell viability (80%) with DNA values that remained stable throughout the culture conditions. Confocal imaging showed clustering of microcapsules in chondrocytes to natriuretic peptide receptor (Npr) 2 and 3. Treatment of cartilage explants with CNP microcapsules led to concentration-dependent inhibition of NO release in response to IL-1β and restoration of matrix synthesis. In summary, we demonstrate controlled delivery of CNP to dampen pro-inflammatory effects induced by IL-1β in cartilage explants. The LbL approach has the potential to promote cartilage repair in vivo.

Original languageEnglish
Pages (from-to)524-531
Number of pages8
JournalBiomacromolecules
Volume16
Issue number2
DOIs
Publication statusPublished - 9 Feb 2015
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Microencapsulation
Drug Compounding
Cartilage
Interleukin-1
Capsules
Chondrocytes
Osteoarthritis
Repair
Natriuretic Peptides
Peptide Receptors
Polyelectrolytes
Encapsulation
Restoration
Cluster Analysis
Cell Survival
Anti-Inflammatory Agents
Cells
DNA
Cytokines

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials
  • Medicine(all)

Cite this

Controlled release of C-type natriuretic peptide by microencapsulation dampens proinflammatory effects induced by IL-1β in cartilage explants. / Peake, Nick J.; Pavlov, Anton M.; D'Souza, Alveena; Pingguan-Murphy, Belinda; Sukhorukov, Gleb B.; Hobbs, Adrian J.; Chowdhury, Tina T.

In: Biomacromolecules, Vol. 16, No. 2, 09.02.2015, p. 524-531.

Research output: Contribution to journalArticle

Peake, Nick J. ; Pavlov, Anton M. ; D'Souza, Alveena ; Pingguan-Murphy, Belinda ; Sukhorukov, Gleb B. ; Hobbs, Adrian J. ; Chowdhury, Tina T. / Controlled release of C-type natriuretic peptide by microencapsulation dampens proinflammatory effects induced by IL-1β in cartilage explants. In: Biomacromolecules. 2015 ; Vol. 16, No. 2. pp. 524-531.
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