Abstract
We examined the role of opioid receptors in the cardioprotective effect of chronic continuous normobaric hypoxia in the model of anoxia/reoxygenation of isolated cardiomyocytes. Adaptation to hypoxia was provided by placing the rats for 21 days in an atmosphere with low O2 content. In intact rats, anoxia/reoxygenation of cardiomyocytes caused death of 23% cells and increased lactate dehydrogenase release from cardiomyocytes. In adapted rats, anoxia/ reoxygenation of cardiomyocytes caused death of only 2.5% cells and the release of lactate dehydrogenase decreased by 25%. Preincubation of the cells with opioid receptor blocker naloxone (300 nM) for 25 min eliminated the adaptive decrease in cell survival and reduction in lactate dehydrogenase release. Hence, opioid receptors of the cardiomyocytes contribute to the cytoprotective effect of chronic normobaric hypoxia.
| Original language | English |
|---|---|
| Article number | A008 |
| Pages (from-to) | 209-212 |
| Number of pages | 4 |
| Journal | Bulletin of Experimental Biology and Medicine |
| Volume | 159 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Jan 2015 |
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Keywords
- Adaptation
- Cardiomyocytes
- Hypoxia
- Opioids
- Reoxygenation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Contribution of opioid receptors to the cytoprotective effect of the adaptation to chronic hypoxia at anoxia/reoxygenation of isolated cardiomyocytes. / Naryzhnaya, N. V.; Maslov, L. N.; Prokudina, E. S.; Lishmanov, Yu B.
In: Bulletin of Experimental Biology and Medicine, Vol. 159, No. 2, A008, 01.01.2015, p. 209-212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Contribution of opioid receptors to the cytoprotective effect of the adaptation to chronic hypoxia at anoxia/reoxygenation of isolated cardiomyocytes
AU - Naryzhnaya, N. V.
AU - Maslov, L. N.
AU - Prokudina, E. S.
AU - Lishmanov, Yu B.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - We examined the role of opioid receptors in the cardioprotective effect of chronic continuous normobaric hypoxia in the model of anoxia/reoxygenation of isolated cardiomyocytes. Adaptation to hypoxia was provided by placing the rats for 21 days in an atmosphere with low O2 content. In intact rats, anoxia/reoxygenation of cardiomyocytes caused death of 23% cells and increased lactate dehydrogenase release from cardiomyocytes. In adapted rats, anoxia/ reoxygenation of cardiomyocytes caused death of only 2.5% cells and the release of lactate dehydrogenase decreased by 25%. Preincubation of the cells with opioid receptor blocker naloxone (300 nM) for 25 min eliminated the adaptive decrease in cell survival and reduction in lactate dehydrogenase release. Hence, opioid receptors of the cardiomyocytes contribute to the cytoprotective effect of chronic normobaric hypoxia.
AB - We examined the role of opioid receptors in the cardioprotective effect of chronic continuous normobaric hypoxia in the model of anoxia/reoxygenation of isolated cardiomyocytes. Adaptation to hypoxia was provided by placing the rats for 21 days in an atmosphere with low O2 content. In intact rats, anoxia/reoxygenation of cardiomyocytes caused death of 23% cells and increased lactate dehydrogenase release from cardiomyocytes. In adapted rats, anoxia/ reoxygenation of cardiomyocytes caused death of only 2.5% cells and the release of lactate dehydrogenase decreased by 25%. Preincubation of the cells with opioid receptor blocker naloxone (300 nM) for 25 min eliminated the adaptive decrease in cell survival and reduction in lactate dehydrogenase release. Hence, opioid receptors of the cardiomyocytes contribute to the cytoprotective effect of chronic normobaric hypoxia.
KW - Adaptation
KW - Cardiomyocytes
KW - Hypoxia
KW - Opioids
KW - Reoxygenation
UR - http://www.scopus.com/inward/record.url?scp=84937000084&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937000084&partnerID=8YFLogxK
U2 - 10.1007/s10517-015-2924-7
DO - 10.1007/s10517-015-2924-7
M3 - Article
C2 - 26085353
AN - SCOPUS:84937000084
VL - 159
SP - 209
EP - 212
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 2
M1 - A008
ER -