Construction of a translation enhancer-containing vector for gene expression in a prokaryotic two-cistron system

Yuri A. Berlin, Klara R. Birikh, Ekaterina N. Lebedenko

Research output: Contribution to journalArticlepeer-review

Abstract

A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.

Original languageEnglish
Pages (from-to)599-605
Number of pages7
JournalNucleosides and Nucleotides
Volume13
Issue number1-3
DOIs
Publication statusPublished - 1 Mar 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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