Construction of a translation enhancer-containing vector for gene expression in a prokaryotic two-cistron system

Abstract

A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.

Original language	English
Pages (from-to)	599-605
Number of pages	7
Journal	Nucleosides and Nucleotides
Volume	13
Issue number	1-3
DOIs	https://doi.org/10.1080/15257779408013265
Publication status	Published - 1 Mar 1994
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Genetics

Access to Document

10.1080/15257779408013265

Fingerprint Dive into the research topics of 'Construction of a translation enhancer-containing vector for gene expression in a prokaryotic two-cistron system'. Together they form a unique fingerprint.

Cite this

@article{66f97f2056754408a926d1a33aeb1df9,

title = "Construction of a translation enhancer-containing vector for gene expression in a prokaryotic two-cistron system",

abstract = "A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.",

author = "Berlin, {Yuri A.} and Birikh, {Klara R.} and Lebedenko, {Ekaterina N.}",

year = "1994",

month = mar,

day = "1",

doi = "10.1080/15257779408013265",

language = "English",

volume = "13",

pages = "599--605",

journal = "Nucleosides and Nucleotides",

issn = "0732-8311",

publisher = "Taylor and Francis Ltd.",

number = "1-3",

}

TY - JOUR

T1 - Construction of a translation enhancer-containing vector for gene expression in a prokaryotic two-cistron system

AU - Berlin, Yuri A.

AU - Birikh, Klara R.

AU - Lebedenko, Ekaterina N.

PY - 1994/3/1

Y1 - 1994/3/1

N2 - A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.

AB - A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.

UR - http://www.scopus.com/inward/record.url?scp=0028331742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028331742&partnerID=8YFLogxK

U2 - 10.1080/15257779408013265

DO - 10.1080/15257779408013265

M3 - Article

AN - SCOPUS:0028331742

VL - 13

SP - 599

EP - 605

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

SN - 0732-8311

IS - 1-3

ER -