TY - JOUR
T1 - Construction of a translation enhancer-containing vector for gene expression in a prokaryotic two-cistron system
AU - Berlin, Yuri A.
AU - Birikh, Klara R.
AU - Lebedenko, Ekaterina N.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1994/3/1
Y1 - 1994/3/1
N2 - A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.
AB - A prokaryotic two-cistron system for Rene expression, based on the pGEM1 plasmid and containing a translation enhancer in the coding part of the first cistron, has been constructed. The gene to be expressed can be inserted into the vector by means of a PCR-mediated approach using type IIS restriction endonucleases (SDL method). Efficiency of the system is exemplified by the expression of a synthetic gene encoding human interleukin la.
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U2 - 10.1080/15257779408013265
DO - 10.1080/15257779408013265
M3 - Article
AN - SCOPUS:0028331742
VL - 13
SP - 599
EP - 605
JO - Nucleosides and Nucleotides
JF - Nucleosides and Nucleotides
SN - 0732-8311
IS - 1-3
ER -