Abstract
In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3h. The RSD of repeatability of 10 consecutive measurements is 1.6% (csarcosine=1.0×10-4moldm-3). Under optimal conditions the calibration dependence was linear in the range 7.5×10-6-5.0×10-4moldm-3 and limit of detection was 2.0×10-6moldm-3.
| Original language | English |
|---|---|
| Pages (from-to) | 2559-2566 |
| Number of pages | 8 |
| Journal | Electroanalysis |
| Volume | 27 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1 Nov 2015 |
| Externally published | Yes |
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Keywords
- Amperometry
- Biosensors
- Electrochemistry
- Sarcosine
- Silver solid amalgam electrode
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine. / Josypčuk, Oksana; Barek, Jiří; Josypčuk, Bohdan.
In: Electroanalysis, Vol. 27, No. 11, 01.11.2015, p. 2559-2566.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine
AU - Josypčuk, Oksana
AU - Barek, Jiří
AU - Josypčuk, Bohdan
PY - 2015/11/1
Y1 - 2015/11/1
N2 - In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3h. The RSD of repeatability of 10 consecutive measurements is 1.6% (csarcosine=1.0×10-4moldm-3). Under optimal conditions the calibration dependence was linear in the range 7.5×10-6-5.0×10-4moldm-3 and limit of detection was 2.0×10-6moldm-3.
AB - In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3h. The RSD of repeatability of 10 consecutive measurements is 1.6% (csarcosine=1.0×10-4moldm-3). Under optimal conditions the calibration dependence was linear in the range 7.5×10-6-5.0×10-4moldm-3 and limit of detection was 2.0×10-6moldm-3.
KW - Amperometry
KW - Biosensors
KW - Electrochemistry
KW - Sarcosine
KW - Silver solid amalgam electrode
UR - http://www.scopus.com/inward/record.url?scp=84954440405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84954440405&partnerID=8YFLogxK
U2 - 10.1002/elan.201500246
DO - 10.1002/elan.201500246
M3 - Article
AN - SCOPUS:84954440405
VL - 27
SP - 2559
EP - 2566
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 11
ER -