Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine

Oksana Josypčuk, Jiří Barek, Bohdan Josypčuk

Research output: Contribution to journal › Article

Abstract

In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3h. The RSD of repeatability of 10 consecutive measurements is 1.6% (c_sarcosine=1.0×10^-4moldm^-3). Under optimal conditions the calibration dependence was linear in the range 7.5×10^-6-5.0×10^-4moldm^-3 and limit of detection was 2.0×10^-6moldm^-3.

Original language	English
Pages (from-to)	2559-2566
Number of pages	8
Journal	Electroanalysis
Volume	27
Issue number	11
DOIs	https://doi.org/10.1002/elan.201500246
Publication status	Published - 1 Nov 2015
Externally published	Yes

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Keywords

Amperometry
Biosensors
Electrochemistry
Sarcosine
Silver solid amalgam electrode

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

Cite this

Josypčuk, O., Barek, J., & Josypčuk, B. (2015). Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine. Electroanalysis, 27(11), 2559-2566. https://doi.org/10.1002/elan.201500246

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10.1002/elan.201500246