Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD

Carla Winkler, Elena N. Atochina-Vasserman, Olaf Holz, Michael F. Beers, Veit J. Erpenbeck, Norbert Krug, Stefan Roepcke, Gereon Lauer, Martin Elmlinger, Jens M. Hohlfeld

Research output: Contribution to journalArticle

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Abstract

Background: Pulmonary surfactant protein D (SP-D) is considered as a candidate biomarker for the functional integrity of the lung and for disease progression, which can be detected in serum. The origin of SP-D in serum and how serum concentrations are related to pulmonary concentrations under inflammatory conditions is still unclear.Methods: In a cross-sectional study comprising non-smokers (n = 10), young - (n = 10), elderly smokers (n = 20), and smokers with COPD (n = 20) we simultaneously analysed pulmonary and serum SP-D levels with regard to pulmonary function, exercise, repeatability and its quaternary structure by native gel electrophoresis. Statistical comparisons were conducted by ANOVA and post-hoc testing for multiple comparisons; repeatability was assessed by Bland-Altman analysis.Results: In COPD, median (IQR) pulmonary SP-D levels were lower (129(68) ng/ml) compared to smokers (young: 299(190), elderly: 296(158) ng/ml; p <0.01) and non-smokers (967(708) ng/ml; p <0.001). The opposite was observed in serum, with higher concentrations in COPD (140(89) ng/ml) as compared to non-smokers (76(47) ng/ml; p <0.01). SP-D levels were reproducible and correlated with the degree of airway obstruction in all smokers. In addition, smoking lead to disruption of the quaternary structure.Conclusions: Pulmonary and serum SP-D levels are stable markers influenced by smoking and related to airflow obstruction and disease state. Smaller subunits of pulmonary SP-D and the rapid increase of serum SP-D levels in COPD due to exercise support the translocation hypothesis and its use as a COPD biomarker.Trial registration: no interventional trial.

Original languageEnglish
Article number29
JournalRespiratory Research
Volume12
DOIs
Publication statusPublished - 11 Mar 2011
Externally publishedYes

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Pulmonary Surfactant-Associated Protein D
Chronic Obstructive Pulmonary Disease
Blood Proteins
Serum
Biomarkers
Smoking
Exercise
Lung
Airway Obstruction
Lung Diseases
Disease Progression
Electrophoresis
Analysis of Variance
Cross-Sectional Studies
Gels

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD. / Winkler, Carla; Atochina-Vasserman, Elena N.; Holz, Olaf; Beers, Michael F.; Erpenbeck, Veit J.; Krug, Norbert; Roepcke, Stefan; Lauer, Gereon; Elmlinger, Martin; Hohlfeld, Jens M.

In: Respiratory Research, Vol. 12, 29, 11.03.2011.

Research output: Contribution to journalArticle

Winkler, C, Atochina-Vasserman, EN, Holz, O, Beers, MF, Erpenbeck, VJ, Krug, N, Roepcke, S, Lauer, G, Elmlinger, M & Hohlfeld, JM 2011, 'Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD', Respiratory Research, vol. 12, 29. https://doi.org/10.1186/1465-9921-12-29
Winkler, Carla ; Atochina-Vasserman, Elena N. ; Holz, Olaf ; Beers, Michael F. ; Erpenbeck, Veit J. ; Krug, Norbert ; Roepcke, Stefan ; Lauer, Gereon ; Elmlinger, Martin ; Hohlfeld, Jens M. / Comprehensive characterisation of pulmonary and serum surfactant protein D in COPD. In: Respiratory Research. 2011 ; Vol. 12.
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AU - Atochina-Vasserman, Elena N.

AU - Holz, Olaf

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AU - Erpenbeck, Veit J.

AU - Krug, Norbert

AU - Roepcke, Stefan

AU - Lauer, Gereon

AU - Elmlinger, Martin

AU - Hohlfeld, Jens M.

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AB - Background: Pulmonary surfactant protein D (SP-D) is considered as a candidate biomarker for the functional integrity of the lung and for disease progression, which can be detected in serum. The origin of SP-D in serum and how serum concentrations are related to pulmonary concentrations under inflammatory conditions is still unclear.Methods: In a cross-sectional study comprising non-smokers (n = 10), young - (n = 10), elderly smokers (n = 20), and smokers with COPD (n = 20) we simultaneously analysed pulmonary and serum SP-D levels with regard to pulmonary function, exercise, repeatability and its quaternary structure by native gel electrophoresis. Statistical comparisons were conducted by ANOVA and post-hoc testing for multiple comparisons; repeatability was assessed by Bland-Altman analysis.Results: In COPD, median (IQR) pulmonary SP-D levels were lower (129(68) ng/ml) compared to smokers (young: 299(190), elderly: 296(158) ng/ml; p <0.01) and non-smokers (967(708) ng/ml; p <0.001). The opposite was observed in serum, with higher concentrations in COPD (140(89) ng/ml) as compared to non-smokers (76(47) ng/ml; p <0.01). SP-D levels were reproducible and correlated with the degree of airway obstruction in all smokers. In addition, smoking lead to disruption of the quaternary structure.Conclusions: Pulmonary and serum SP-D levels are stable markers influenced by smoking and related to airflow obstruction and disease state. Smaller subunits of pulmonary SP-D and the rapid increase of serum SP-D levels in COPD due to exercise support the translocation hypothesis and its use as a COPD biomarker.Trial registration: no interventional trial.

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