Composite SERS-based satellites navigated by optical tweezers for single cell analysis

Abstract

Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

Original language	English
Pages (from-to)	4981-4986
Number of pages	6
Journal	The Analyst
Volume	140
Issue number	15
DOIs	https://doi.org/10.1039/c5an00392j
Publication status	Published - 5 Feb 2015
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Electrochemistry
Biochemistry
Environmental Chemistry
Medicine(all)

Access to Document

10.1039/c5an00392j

Fingerprint Dive into the research topics of 'Composite SERS-based satellites navigated by optical tweezers for single cell analysis'. Together they form a unique fingerprint.

Cite this

Composite SERS-based satellites navigated by optical tweezers for single cell analysis. / Stetciura, Inna Y.; Yashchenok, Alexey; Masic, Admir; Lyubin, Evgeny V.; Inozemtseva, Olga A.; Drozdova, Maria G.; Markvichova, Elena A.; Khlebtsov, Boris N.; Fedyanin, Andrey A.; Sukhorukov, Gleb B.; Gorin, Dmitry A.; Volodkin, Dmitry.

In: The Analyst, Vol. 140, No. 15, 05.02.2015, p. 4981-4986.

Research output: Contribution to journal › Article › peer-review

Stetciura, Inna Y. ; Yashchenok, Alexey ; Masic, Admir ; Lyubin, Evgeny V. ; Inozemtseva, Olga A. ; Drozdova, Maria G. ; Markvichova, Elena A. ; Khlebtsov, Boris N. ; Fedyanin, Andrey A. ; Sukhorukov, Gleb B. ; Gorin, Dmitry A. ; Volodkin, Dmitry. / Composite SERS-based satellites navigated by optical tweezers for single cell analysis. In: The Analyst. 2015 ; Vol. 140, No. 15. pp. 4981-4986.

@article{8b7a1aa961e845ad8a3e43a443fb292b,

title = "Composite SERS-based satellites navigated by optical tweezers for single cell analysis",

abstract = "Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.",

author = "Stetciura, {Inna Y.} and Alexey Yashchenok and Admir Masic and Lyubin, {Evgeny V.} and Inozemtseva, {Olga A.} and Drozdova, {Maria G.} and Markvichova, {Elena A.} and Khlebtsov, {Boris N.} and Fedyanin, {Andrey A.} and Sukhorukov, {Gleb B.} and Gorin, {Dmitry A.} and Dmitry Volodkin",

year = "2015",

month = feb,

day = "5",

doi = "10.1039/c5an00392j",

language = "English",

volume = "140",

pages = "4981--4986",

journal = "The Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "15",

}

TY - JOUR

T1 - Composite SERS-based satellites navigated by optical tweezers for single cell analysis

AU - Stetciura, Inna Y.

AU - Yashchenok, Alexey

AU - Masic, Admir

AU - Lyubin, Evgeny V.

AU - Inozemtseva, Olga A.

AU - Drozdova, Maria G.

AU - Markvichova, Elena A.

AU - Khlebtsov, Boris N.

AU - Fedyanin, Andrey A.

AU - Sukhorukov, Gleb B.

AU - Gorin, Dmitry A.

AU - Volodkin, Dmitry

PY - 2015/2/5

Y1 - 2015/2/5

N2 - Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

AB - Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

UR - http://www.scopus.com/inward/record.url?scp=84951826091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951826091&partnerID=8YFLogxK

U2 - 10.1039/c5an00392j

DO - 10.1039/c5an00392j

M3 - Article

C2 - 26040199

AN - SCOPUS:84951826091

VL - 140

SP - 4981

EP - 4986

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 15

ER -