Abstract
Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.
Original language | English |
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Pages (from-to) | 4981-4986 |
Number of pages | 6 |
Journal | The Analyst |
Volume | 140 |
Issue number | 15 |
DOIs | |
Publication status | Published - 5 Feb 2015 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Electrochemistry
- Biochemistry
- Environmental Chemistry
- Medicine(all)