Composite SERS-based satellites navigated by optical tweezers for single cell analysis

Inna Y. Stetciura, Alexey Yashchenok, Admir Masic, Evgeny V. Lyubin, Olga A. Inozemtseva, Maria G. Drozdova, Elena A. Markvichova, Boris N. Khlebtsov, Andrey A. Fedyanin, Gleb B. Sukhorukov, Dmitry A. Gorin, Dmitry Volodkin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

Original languageEnglish
Pages (from-to)4981-4986
Number of pages6
JournalThe Analyst
Volume140
Issue number15
DOIs
Publication statusPublished - 5 Feb 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry
  • Medicine(all)

Cite this

Stetciura, I. Y., Yashchenok, A., Masic, A., Lyubin, E. V., Inozemtseva, O. A., Drozdova, M. G., Markvichova, E. A., Khlebtsov, B. N., Fedyanin, A. A., Sukhorukov, G. B., Gorin, D. A., & Volodkin, D. (2015). Composite SERS-based satellites navigated by optical tweezers for single cell analysis. The Analyst, 140(15), 4981-4986. https://doi.org/10.1039/c5an00392j