Composite SERS-based satellites navigated by optical tweezers for single cell analysis

Abstract

Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

Original language	English
Pages (from-to)	4981-4986
Number of pages	6
Journal	The Analyst
Volume	140
Issue number	15
DOIs	https://doi.org/10.1039/c5an00392j
Publication status	Published - 5 Feb 2015
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Electrochemistry
Biochemistry
Environmental Chemistry
Medicine(all)

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10.1039/c5an00392j

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Composite SERS-based satellites navigated by optical tweezers for single cell analysis. / Stetciura, Inna Y.; Yashchenok, Alexey; Masic, Admir; Lyubin, Evgeny V.; Inozemtseva, Olga A.; Drozdova, Maria G.; Markvichova, Elena A.; Khlebtsov, Boris N.; Fedyanin, Andrey A.; Sukhorukov, Gleb B.; Gorin, Dmitry A.; Volodkin, Dmitry.

In: The Analyst, Vol. 140, No. 15, 05.02.2015, p. 4981-4986.

Research output: Contribution to journal › Article › peer-review

Stetciura, Inna Y. ; Yashchenok, Alexey ; Masic, Admir ; Lyubin, Evgeny V. ; Inozemtseva, Olga A. ; Drozdova, Maria G. ; Markvichova, Elena A. ; Khlebtsov, Boris N. ; Fedyanin, Andrey A. ; Sukhorukov, Gleb B. ; Gorin, Dmitry A. ; Volodkin, Dmitry. / Composite SERS-based satellites navigated by optical tweezers for single cell analysis. In: The Analyst. 2015 ; Vol. 140, No. 15. pp. 4981-4986.

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abstract = "Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.",

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AU - Stetciura, Inna Y.

AU - Yashchenok, Alexey

AU - Masic, Admir

AU - Lyubin, Evgeny V.

AU - Inozemtseva, Olga A.

AU - Drozdova, Maria G.

AU - Markvichova, Elena A.

AU - Khlebtsov, Boris N.

AU - Fedyanin, Andrey A.

AU - Sukhorukov, Gleb B.

AU - Gorin, Dmitry A.

AU - Volodkin, Dmitry

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AB - Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

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Composite SERS-based satellites navigated by optical tweezers for single cell analysis

Abstract

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