Abstract
Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.
| Original language | English |
|---|---|
| Pages (from-to) | 4981-4986 |
| Number of pages | 6 |
| Journal | The Analyst |
| Volume | 140 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - 5 Feb 2015 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Electrochemistry
- Biochemistry
- Environmental Chemistry
- Medicine(all)
Cite this
Composite SERS-based satellites navigated by optical tweezers for single cell analysis. / Stetciura, Inna Y.; Yashchenok, Alexey; Masic, Admir; Lyubin, Evgeny V.; Inozemtseva, Olga A.; Drozdova, Maria G.; Markvichova, Elena A.; Khlebtsov, Boris N.; Fedyanin, Andrey A.; Sukhorukov, Gleb B.; Gorin, Dmitry A.; Volodkin, Dmitry.
In: The Analyst, Vol. 140, No. 15, 05.02.2015, p. 4981-4986.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Composite SERS-based satellites navigated by optical tweezers for single cell analysis
AU - Stetciura, Inna Y.
AU - Yashchenok, Alexey
AU - Masic, Admir
AU - Lyubin, Evgeny V.
AU - Inozemtseva, Olga A.
AU - Drozdova, Maria G.
AU - Markvichova, Elena A.
AU - Khlebtsov, Boris N.
AU - Fedyanin, Andrey A.
AU - Sukhorukov, Gleb B.
AU - Gorin, Dmitry A.
AU - Volodkin, Dmitry
PY - 2015/2/5
Y1 - 2015/2/5
N2 - Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.
AB - Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.
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UR - http://www.scopus.com/inward/citedby.url?scp=84951826091&partnerID=8YFLogxK
U2 - 10.1039/c5an00392j
DO - 10.1039/c5an00392j
M3 - Article
VL - 140
SP - 4981
EP - 4986
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 15
ER -