Composite SERS-based satellites navigated by optical tweezers for single cell analysis

Inna Y. Stetciura, Alexey Yashchenok, Admir Masic, Evgeny V. Lyubin, Olga A. Inozemtseva, Maria G. Drozdova, Elena A. Markvichova, Boris N. Khlebtsov, Andrey A. Fedyanin, Gleb B. Sukhorukov, Dmitry A. Gorin, Dmitry Volodkin

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

Original language	English
Pages (from-to)	4981-4986
Number of pages	6
Journal	The Analyst
Volume	140
Issue number	15
DOIs	https://doi.org/10.1039/c5an00392j
Publication status	Published - 5 Feb 2015
Externally published	Yes

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ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Electrochemistry
Biochemistry
Environmental Chemistry
Medicine(all)

Cite this

Stetciura, I. Y., Yashchenok, A., Masic, A., Lyubin, E. V., Inozemtseva, O. A., Drozdova, M. G., Markvichova, E. A., Khlebtsov, B. N., Fedyanin, A. A., Sukhorukov, G. B., Gorin, D. A., & Volodkin, D. (2015). Composite SERS-based satellites navigated by optical tweezers for single cell analysis. The Analyst, 140(15), 4981-4986. https://doi.org/10.1039/c5an00392j

Composite SERS-based satellites navigated by optical tweezers for single cell analysis. / Stetciura, Inna Y.; Yashchenok, Alexey; Masic, Admir; Lyubin, Evgeny V.; Inozemtseva, Olga A.; Drozdova, Maria G.; Markvichova, Elena A.; Khlebtsov, Boris N.; Fedyanin, Andrey A.; Sukhorukov, Gleb B.; Gorin, Dmitry A.; Volodkin, Dmitry.

In: The Analyst, Vol. 140, No. 15, 05.02.2015, p. 4981-4986.

Research output: Contribution to journal › Article

Stetciura, Inna Y. ; Yashchenok, Alexey ; Masic, Admir ; Lyubin, Evgeny V. ; Inozemtseva, Olga A. ; Drozdova, Maria G. ; Markvichova, Elena A. ; Khlebtsov, Boris N. ; Fedyanin, Andrey A. ; Sukhorukov, Gleb B. ; Gorin, Dmitry A. ; Volodkin, Dmitry. / Composite SERS-based satellites navigated by optical tweezers for single cell analysis. In: The Analyst. 2015 ; Vol. 140, No. 15. pp. 4981-4986.

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Access to Document

10.1039/c5an00392j