Abstract
In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.
| Original language | English |
|---|---|
| Title of host publication | IEEE-NANO 2015 - 15th International Conference on Nanotechnology |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 13-16 |
| Number of pages | 4 |
| ISBN (Electronic) | 9781467381550 |
| DOIs | |
| Publication status | Published - 2015 |
| Externally published | Yes |
| Event | 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy Duration: 27 Jul 2015 → 30 Jul 2015 |
Conference
| Conference | 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 |
|---|---|
| Country | Italy |
| City | Rome |
| Period | 27.7.15 → 30.7.15 |
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Keywords
- cancer cells
- magnetic particles
- scFv antibody
- theranostic
ASJC Scopus subject areas
- Process Chemistry and Technology
- Electrical and Electronic Engineering
- Ceramics and Composites
- Electronic, Optical and Magnetic Materials
- Surfaces, Coatings and Films
Cite this
Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. / Shipunova, Victoria O.; Nikitin, Maxim P.; Mironova, Kristina E.; Deyev, Sergey M.; Nikitin, Petr I.
IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. p. 13-16 7388956.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells
AU - Shipunova, Victoria O.
AU - Nikitin, Maxim P.
AU - Mironova, Kristina E.
AU - Deyev, Sergey M.
AU - Nikitin, Petr I.
PY - 2015
Y1 - 2015
N2 - In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.
AB - In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.
KW - cancer cells
KW - magnetic particles
KW - scFv antibody
KW - theranostic
UR - http://www.scopus.com/inward/record.url?scp=84964374794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964374794&partnerID=8YFLogxK
U2 - 10.1109/NANO.2015.7388956
DO - 10.1109/NANO.2015.7388956
M3 - Conference contribution
SP - 13
EP - 16
BT - IEEE-NANO 2015 - 15th International Conference on Nanotechnology
PB - Institute of Electrical and Electronics Engineers Inc.
ER -