Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells

Victoria O. Shipunova, Maxim P. Nikitin, Kristina E. Mironova, Sergey M. Deyev, Petr I. Nikitin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.

Original languageEnglish
Title of host publicationIEEE-NANO 2015 - 15th International Conference on Nanotechnology
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages13-16
Number of pages4
ISBN (Electronic)9781467381550
DOIs
Publication statusPublished - 2015
Externally publishedYes
Event15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy
Duration: 27 Jul 201530 Jul 2015

Conference

Conference15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
CountryItaly
CityRome
Period27.7.1530.7.15

Fingerprint

Single-Chain Antibodies
Antibodies
Cells
Nanoparticles
Proteins
Glues
Fusion reactions

Keywords

  • cancer cells
  • magnetic particles
  • scFv antibody
  • theranostic

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Electrical and Electronic Engineering
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films

Cite this

Shipunova, V. O., Nikitin, M. P., Mironova, K. E., Deyev, S. M., & Nikitin, P. I. (2015). Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology (pp. 13-16). [7388956] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2015.7388956

Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. / Shipunova, Victoria O.; Nikitin, Maxim P.; Mironova, Kristina E.; Deyev, Sergey M.; Nikitin, Petr I.

IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. p. 13-16 7388956.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shipunova, VO, Nikitin, MP, Mironova, KE, Deyev, SM & Nikitin, PI 2015, Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. in IEEE-NANO 2015 - 15th International Conference on Nanotechnology., 7388956, Institute of Electrical and Electronics Engineers Inc., pp. 13-16, 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015, Rome, Italy, 27.7.15. https://doi.org/10.1109/NANO.2015.7388956
Shipunova VO, Nikitin MP, Mironova KE, Deyev SM, Nikitin PI. Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc. 2015. p. 13-16. 7388956 https://doi.org/10.1109/NANO.2015.7388956
Shipunova, Victoria O. ; Nikitin, Maxim P. ; Mironova, Kristina E. ; Deyev, Sergey M. ; Nikitin, Petr I. / Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 13-16
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