Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells

Abstract

In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.

Original language	English
Title of host publication	IEEE-NANO 2015 - 15th International Conference on Nanotechnology
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	13-16
Number of pages	4
ISBN (Electronic)	9781467381550
DOIs	https://doi.org/10.1109/NANO.2015.7388956
Publication status	Published - 2015
Externally published	Yes
Event	15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy Duration: 27 Jul 2015 → 30 Jul 2015

Conference

Conference	15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
Country	Italy
City	Rome
Period	27.7.15 → 30.7.15

Keywords

cancer cells
magnetic particles
scFv antibody
theranostic

ASJC Scopus subject areas

Process Chemistry and Technology
Electrical and Electronic Engineering
Ceramics and Composites
Electronic, Optical and Magnetic Materials
Surfaces, Coatings and Films

Access to Document

10.1109/NANO.2015.7388956

Fingerprint Dive into the research topics of 'Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells'. Together they form a unique fingerprint.

Cite this

Shipunova, V. O., Nikitin, M. P., Mironova, K. E., Deyev, S. M., & Nikitin, P. I. (2015). Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology (pp. 13-16). [7388956] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2015.7388956

Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. / Shipunova, Victoria O.; Nikitin, Maxim P.; Mironova, Kristina E.; Deyev, Sergey M.; Nikitin, Petr I.

IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. p. 13-16 7388956.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shipunova, VO, Nikitin, MP, Mironova, KE, Deyev, SM & Nikitin, PI 2015, Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells. in IEEE-NANO 2015 - 15th International Conference on Nanotechnology., 7388956, Institute of Electrical and Electronics Engineers Inc., pp. 13-16, 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015, Rome, Italy, 27.7.15. https://doi.org/10.1109/NANO.2015.7388956

@inproceedings{1bae95f52108454abc3ca31ed4d88fc7,

title = "Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells",

abstract = "In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.",

keywords = "cancer cells, magnetic particles, scFv antibody, theranostic",

author = "Shipunova, {Victoria O.} and Nikitin, {Maxim P.} and Mironova, {Kristina E.} and Deyev, {Sergey M.} and Nikitin, {Petr I.}",

year = "2015",

doi = "10.1109/NANO.2015.7388956",

language = "English",

pages = "13--16",

booktitle = "IEEE-NANO 2015 - 15th International Conference on Nanotechnology",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 ; Conference date: 27-07-2015 Through 30-07-2015",

}

TY - GEN

T1 - Complexes of magnetic nanoparticles and scFv antibodies for targeting and visualizing cancer cells

AU - Shipunova, Victoria O.

AU - Nikitin, Maxim P.

AU - Mironova, Kristina E.

AU - Deyev, Sergey M.

AU - Nikitin, Petr I.

PY - 2015

Y1 - 2015

N2 - In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.

AB - In this work we describe a design of multifunctional complexes based on magnetic nanoparticles and anti-cancer mini-antibodies of scFv format that selectively recognize oncomarker HER2/neu on the surface of human breast adenocarcinoma cells. ScFv, having small size and being fully genetically encoded, offer significant advantages over full size antibody for targeted delivery of nanoparticles both in vitro and in vivo, such as low cross-reactivity and immunogenicity and ease of biotechnological production in prokaryotic expression systems. These structures were obtained through the use of the barnase, barstar protein pair as a molecular glue between nanoparticles and recognizing antibodies. Moreover, for demonstrating the ability of obtaining a high diversity of scFv and magnetic nanoparticles-based constructions, we obtained complexes of magnetic nanoparticles and fusion protein of anti-HER2/neu mini-antibody 4D5scFv and fluorescent protein mCherry that specifically stained HER2/neu positive cells. Application of such targeted nanoparticles opens up new possibilities for designing new effective methods of cancer diagnostics.

KW - cancer cells

KW - magnetic particles

KW - scFv antibody

KW - theranostic

UR - http://www.scopus.com/inward/record.url?scp=84964374794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964374794&partnerID=8YFLogxK

U2 - 10.1109/NANO.2015.7388956

DO - 10.1109/NANO.2015.7388956

M3 - Conference contribution

AN - SCOPUS:84964374794

SP - 13

EP - 16

BT - IEEE-NANO 2015 - 15th International Conference on Nanotechnology

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015

Y2 - 27 July 2015 through 30 July 2015

ER -