Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines

K. Juarez-Moreno, E. B. Gonzalez, N. Girón-Vazquez, R. A. Chávez-Santoscoy, J. D. Mota-Morales, L. L. Perez-Mozqueda, M. R. Garcia-Garcia, A. Pestryakov, N. Bogdanchikova

Research output: Contribution to journalArticle

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Abstract

The wide application of silver nanoparticles (AgNPs) has pointed out the need to evaluate their potential risk and toxic effects on human health. Herein, the cytotoxic effects of Argovit™ AgNPs were evaluated on eight cancer cell lines. Further cytotoxic studies were performed in gynecological cancer cell lines from cervical (HeLa) and breast (MDA-MB-231 and MCF7) cancer. In both cases, the half maximal inhibitory concentration (IC50) of AgNPs produced the formation of reactive oxygen species (ROS) after 24 h of incubation, but it was not statistically significant compared with untreated cells. However, HeLa, MDA-MB-231, and MCF7 cells treated with the maximal IC of AgNPs induced the formation of ROS either at 12 or 24 h of incubation. Genotoxicity achieved by comet assay in HeLa, MDA-MB-231, and MCF7 cells revealed that exposure to IC50 of AgNPs does not induced noticeable DNA damage in the cells. However, the IC of AgNPs provoked severe DNA damage after 12 and 24 h of exposure. We conclude that, Argovit (polyvinylpyrrolidone-coated AgNPs) induce a cytotoxic effect in a time and dose-dependent manner in all the eight cancer cell lines tested. Nevertheless, the genotoxic effect is mainly restricted by the concentration effect. The results contribute to explore new therapeutic applications of AgNPs for malignances in murine models and to study in deep the cytotoxic and genotoxic effects of AgNPs in healthy cells at the surrounding tissue of the neoplasia.

Original languageEnglish
Pages (from-to)931-948
Number of pages18
JournalHuman and Experimental Toxicology
Volume36
Issue number9
DOIs
Publication statusPublished - 1 Sep 2017

Fingerprint

Cytotoxicity
Silver
Uterine Cervical Neoplasms
Nanoparticles
Cells
Breast Neoplasms
Cell Line
MCF-7 Cells
Reactive Oxygen Species
Neoplasms
Inhibitory Concentration 50
DNA Damage
Povidone
Poisons
DNA
Comet Assay
Assays
Health
Tissue
Breast

Keywords

  • cancer
  • cytotoxicity
  • genotoxicity
  • nanobiotechnology
  • nanotoxicology
  • Silver nanoparticles
  • triple-negative breast cancer

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Juarez-Moreno, K., Gonzalez, E. B., Girón-Vazquez, N., Chávez-Santoscoy, R. A., Mota-Morales, J. D., Perez-Mozqueda, L. L., ... Bogdanchikova, N. (2017). Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines. Human and Experimental Toxicology, 36(9), 931-948. https://doi.org/10.1177/0960327116675206

Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines. / Juarez-Moreno, K.; Gonzalez, E. B.; Girón-Vazquez, N.; Chávez-Santoscoy, R. A.; Mota-Morales, J. D.; Perez-Mozqueda, L. L.; Garcia-Garcia, M. R.; Pestryakov, A.; Bogdanchikova, N.

In: Human and Experimental Toxicology, Vol. 36, No. 9, 01.09.2017, p. 931-948.

Research output: Contribution to journalArticle

Juarez-Moreno, K, Gonzalez, EB, Girón-Vazquez, N, Chávez-Santoscoy, RA, Mota-Morales, JD, Perez-Mozqueda, LL, Garcia-Garcia, MR, Pestryakov, A & Bogdanchikova, N 2017, 'Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines', Human and Experimental Toxicology, vol. 36, no. 9, pp. 931-948. https://doi.org/10.1177/0960327116675206
Juarez-Moreno K, Gonzalez EB, Girón-Vazquez N, Chávez-Santoscoy RA, Mota-Morales JD, Perez-Mozqueda LL et al. Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines. Human and Experimental Toxicology. 2017 Sep 1;36(9):931-948. https://doi.org/10.1177/0960327116675206
Juarez-Moreno, K. ; Gonzalez, E. B. ; Girón-Vazquez, N. ; Chávez-Santoscoy, R. A. ; Mota-Morales, J. D. ; Perez-Mozqueda, L. L. ; Garcia-Garcia, M. R. ; Pestryakov, A. ; Bogdanchikova, N. / Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines. In: Human and Experimental Toxicology. 2017 ; Vol. 36, No. 9. pp. 931-948.
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