Comparing the measured affinity of 111In-labeled ligands for cellular receptors by monitoring gamma, beta, or X-ray radiation with three different LigandTracer® devices

Zohreh Varasteh, Anna Orlova

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

LigandTracer instruments measuring high energy photons (gamma-rays), particles (electrons and positrons) and low energy photons (X-rays) are novel semi-automated devices which produce real-time radiotracer-receptor interaction data for measurement of the affinity. The affinities of two different 111In-labeled ligands were measured in three different LigandTracer devices detecting high energy gamma, Auger and internal conversion electrons, or X-rays. The obtained similar uptake/retention patterns (binding curves) show that even at low signal-to-noise ratios (data from Auger and internal conversion electrons, and X-rays), reliable binding data can be captured. This verifies that different devices could be used as alternatives for kinetic evaluation studies for radionuclides emitting different type of radiation.

Original languageEnglish
Pages (from-to)823-828
Number of pages6
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume304
Issue number2
DOIs
Publication statusPublished - 1 May 2015
Externally publishedYes

Keywords

  • In
  • Affinity
  • Binding kinetics
  • LigandTracer Grey
  • LigandTracer White
  • LigandTracer Yellow

ASJC Scopus subject areas

  • Analytical Chemistry
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Pollution
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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