Abstract
Objective: There is plenty of information dedicated to nanomaterial toxicity, but it is often contradictory. This work presents results of the comparative investigation of silver and gold nanoparticles genotoxicity and genotoxic effect of water medium after the spark dispersion process. Material and methods: The nanoparticles were produced in water by the modified energy-efficient electric spark dispersion method. The comet assay, transmission electron microscopy, DLS spectroscopy and thermal desorption of nitrogen (BET method) were used for the investigation. Results: The silver nanoparticles showed a genotoxic effect which appeared at concentration of 0.03 mg/ml and above. The application of gold nanoparticles did not lead to a significant DNA damage at concentrations range of 0.01 - 0.03 mg/ml. However, a notable level of genotoxicity was observed at concentrations of gold nanoparticles about 0.1 mg/ml. Supernatant water medium (with trace of Au and Ag metals) following the spark dispersion process showed no genotoxic action. Conclusion: Accordingly, the testing of the nanoparticles produced by the electric spark dispersion in water revealed more expressed genotoxic effect of silver nanoparticles in comparison with the gold ones.
| Original language | English |
|---|---|
| Pages (from-to) | 35-39 |
| Number of pages | 5 |
| Journal | Journal of Applied Pharmaceutical Science |
| Volume | 7 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jul 2017 |
Fingerprint
Keywords
- Comet assay
- Electric spark dispersion
- Genotoxicity
- Gold nanoparticles
- Leukocytes
- Silver nanoparticles
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmacology (medical)
Cite this
Comparative study of genotoxicity of silver and gold nanoparticles prepared by the electric spark dispersion method. / Plotnikov, Evgenii; Zhuravkov, Sergey; Gapeyev, Andrew; Plotnikov, Vladimir; Martemianova, Irina; Martemianov, Dmitrii.
In: Journal of Applied Pharmaceutical Science, Vol. 7, No. 7, 01.07.2017, p. 35-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative study of genotoxicity of silver and gold nanoparticles prepared by the electric spark dispersion method
AU - Plotnikov, Evgenii
AU - Zhuravkov, Sergey
AU - Gapeyev, Andrew
AU - Plotnikov, Vladimir
AU - Martemianova, Irina
AU - Martemianov, Dmitrii
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Objective: There is plenty of information dedicated to nanomaterial toxicity, but it is often contradictory. This work presents results of the comparative investigation of silver and gold nanoparticles genotoxicity and genotoxic effect of water medium after the spark dispersion process. Material and methods: The nanoparticles were produced in water by the modified energy-efficient electric spark dispersion method. The comet assay, transmission electron microscopy, DLS spectroscopy and thermal desorption of nitrogen (BET method) were used for the investigation. Results: The silver nanoparticles showed a genotoxic effect which appeared at concentration of 0.03 mg/ml and above. The application of gold nanoparticles did not lead to a significant DNA damage at concentrations range of 0.01 - 0.03 mg/ml. However, a notable level of genotoxicity was observed at concentrations of gold nanoparticles about 0.1 mg/ml. Supernatant water medium (with trace of Au and Ag metals) following the spark dispersion process showed no genotoxic action. Conclusion: Accordingly, the testing of the nanoparticles produced by the electric spark dispersion in water revealed more expressed genotoxic effect of silver nanoparticles in comparison with the gold ones.
AB - Objective: There is plenty of information dedicated to nanomaterial toxicity, but it is often contradictory. This work presents results of the comparative investigation of silver and gold nanoparticles genotoxicity and genotoxic effect of water medium after the spark dispersion process. Material and methods: The nanoparticles were produced in water by the modified energy-efficient electric spark dispersion method. The comet assay, transmission electron microscopy, DLS spectroscopy and thermal desorption of nitrogen (BET method) were used for the investigation. Results: The silver nanoparticles showed a genotoxic effect which appeared at concentration of 0.03 mg/ml and above. The application of gold nanoparticles did not lead to a significant DNA damage at concentrations range of 0.01 - 0.03 mg/ml. However, a notable level of genotoxicity was observed at concentrations of gold nanoparticles about 0.1 mg/ml. Supernatant water medium (with trace of Au and Ag metals) following the spark dispersion process showed no genotoxic action. Conclusion: Accordingly, the testing of the nanoparticles produced by the electric spark dispersion in water revealed more expressed genotoxic effect of silver nanoparticles in comparison with the gold ones.
KW - Comet assay
KW - Electric spark dispersion
KW - Genotoxicity
KW - Gold nanoparticles
KW - Leukocytes
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85026644514&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026644514&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2017.70705
DO - 10.7324/JAPS.2017.70705
M3 - Article
VL - 7
SP - 35
EP - 39
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
SN - 2231-3354
IS - 7
ER -