Comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of saussurea controversa

Igor Khlusov, Elena Avdeeva, Valeria Shupletsova, Olga Khaziakhmatova, Larisa Litvinova, Ekaterina Porokhova, Yaroslav Reshetov, Irina Zvereva, Lyudmila Mushtovatova, Mariia Karpova, Artem Guryev, Irina Sukhodolo, Mikhail Belousov

Research output: Contribution to journalArticle

Abstract

The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC>F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.

Original languageEnglish
Article numbermolecules24203680
JournalMolecules
Volume24
Issue number20
DOIs
Publication statusPublished - 12 Oct 2019

Fingerprint

Saussurea
Uronic Acids
Rhamnose
Arabinose
polysaccharides
Osteoblasts
Quercetin
Cytotoxicity
Mannose
Glycosides
Mesenchymal Stromal Cells
Galactose
Flavonoids
leaves
Polysaccharides
Bone
evaluation
Bone Fractures
prophylaxis
Pharmaceutical Preparations

Keywords

  • Alizarin red staining
  • Cytotoxicity
  • Flavonoids
  • Human adiposederived multipotent mesenchymal stromal cells
  • Polysaccharides
  • Saussurea controversa DC (Asteraceae)
  • Staphylococcus aureus growth

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of saussurea controversa. / Khlusov, Igor; Avdeeva, Elena; Shupletsova, Valeria; Khaziakhmatova, Olga; Litvinova, Larisa; Porokhova, Ekaterina; Reshetov, Yaroslav; Zvereva, Irina; Mushtovatova, Lyudmila; Karpova, Mariia; Guryev, Artem; Sukhodolo, Irina; Belousov, Mikhail.

In: Molecules, Vol. 24, No. 20, molecules24203680, 12.10.2019.

Research output: Contribution to journalArticle

Khlusov, I, Avdeeva, E, Shupletsova, V, Khaziakhmatova, O, Litvinova, L, Porokhova, E, Reshetov, Y, Zvereva, I, Mushtovatova, L, Karpova, M, Guryev, A, Sukhodolo, I & Belousov, M 2019, 'Comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of saussurea controversa', Molecules, vol. 24, no. 20, molecules24203680. https://doi.org/10.3390/molecules24203680
Khlusov, Igor ; Avdeeva, Elena ; Shupletsova, Valeria ; Khaziakhmatova, Olga ; Litvinova, Larisa ; Porokhova, Ekaterina ; Reshetov, Yaroslav ; Zvereva, Irina ; Mushtovatova, Lyudmila ; Karpova, Mariia ; Guryev, Artem ; Sukhodolo, Irina ; Belousov, Mikhail. / Comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of saussurea controversa. In: Molecules. 2019 ; Vol. 24, No. 20.
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AU - Khaziakhmatova, Olga

AU - Litvinova, Larisa

AU - Porokhova, Ekaterina

AU - Reshetov, Yaroslav

AU - Zvereva, Irina

AU - Mushtovatova, Lyudmila

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