Abstract
The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC>F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.
| Original language | English |
|---|---|
| Article number | molecules24203680 |
| Journal | Molecules |
| Volume | 24 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 12 Oct 2019 |
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Keywords
- Alizarin red staining
- Cytotoxicity
- Flavonoids
- Human adiposederived multipotent mesenchymal stromal cells
- Polysaccharides
- Saussurea controversa DC (Asteraceae)
- Staphylococcus aureus growth
ASJC Scopus subject areas
- Analytical Chemistry
- Chemistry (miscellaneous)
- Molecular Medicine
- Pharmaceutical Science
- Drug Discovery
- Physical and Theoretical Chemistry
- Organic Chemistry
Cite this
Comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of saussurea controversa. / Khlusov, Igor; Avdeeva, Elena; Shupletsova, Valeria; Khaziakhmatova, Olga; Litvinova, Larisa; Porokhova, Ekaterina; Reshetov, Yaroslav; Zvereva, Irina; Mushtovatova, Lyudmila; Karpova, Mariia; Guryev, Artem; Sukhodolo, Irina; Belousov, Mikhail.
In: Molecules, Vol. 24, No. 20, molecules24203680, 12.10.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative in vitro evaluation of antibacterial and osteogenic activity of polysaccharide and flavonoid fractions isolated from the leaves of saussurea controversa
AU - Khlusov, Igor
AU - Avdeeva, Elena
AU - Shupletsova, Valeria
AU - Khaziakhmatova, Olga
AU - Litvinova, Larisa
AU - Porokhova, Ekaterina
AU - Reshetov, Yaroslav
AU - Zvereva, Irina
AU - Mushtovatova, Lyudmila
AU - Karpova, Mariia
AU - Guryev, Artem
AU - Sukhodolo, Irina
AU - Belousov, Mikhail
PY - 2019/10/12
Y1 - 2019/10/12
N2 - The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC>F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.
AB - The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC>F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.
KW - Alizarin red staining
KW - Cytotoxicity
KW - Flavonoids
KW - Human adiposederived multipotent mesenchymal stromal cells
KW - Polysaccharides
KW - Saussurea controversa DC (Asteraceae)
KW - Staphylococcus aureus growth
UR - http://www.scopus.com/inward/record.url?scp=85073427849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073427849&partnerID=8YFLogxK
U2 - 10.3390/molecules24203680
DO - 10.3390/molecules24203680
M3 - Article
C2 - 31614835
AN - SCOPUS:85073427849
VL - 24
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 20
M1 - molecules24203680
ER -