Colony-forming activity of unipotent hemopoietic precursors under the effect of nanosized ferrites in a constant magnetic field in vitro

Igor Albertovich Khlusov, L. V. Zagrebin, S. S. Shestov, V. I. Itin, V. S. Sedoi, T. A. Feduschak, O. G. Terekhova, A. A. Magaeva, E. P. Naiden, S. A. Antipov, E. S. Puchkovskaya, G. B. Slepchenko, G. T. Sukhikh

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Abstract

We studied in vitro effect of ferrimagnetic nanoparticles in a dose of 3 mg/liter (10 maximum permissible concentrations) on colony-forming capacity of bone marrow granulocytic and monocytic precursors in a constant magnetic field at magnetic field intensity of 200 Oe. We tested powders obtained by the methods of electrical explosion of conductors (magnetite and a mixture of hematite with magnetite) or mechanochemical synthesis (cobalt ferrite). According to electron microscopy, size of particles was within 6-65 nm. Specific effect of nanopowders on functional properties of hemopoietic and stromal cells were demonstrated; this effect was not related to dissolution of these powders, but had a complex nature. It depends on the size and magnetic characteristics of powder particles, the route and dose of administration, and the presence of external magnetic field. It was emphasized that in multicellular systems a reaction of committed hemopoietic precursors mediated via cells (factors) of microenvironment cannot be excluded, the state of this system varying in different individuals and under different conditions. Our data open new vistas for the creation and targeted use of nanosized materials and technologies for individual therapy in the context of personalized medicine.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalBulletin of Experimental Biology and Medicine
Volume145
Issue number1
DOIs
Publication statusPublished - 11 Aug 2008

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Keywords

  • Fibroblasts
  • Granulocytic and monocytic precursors
  • Magnetosensitive iron oxides
  • Mouse bone marrow

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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