CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex

Yury Kostyukevich, Aleksej A. Shulga, Alexey Kononikhin, Igor Popov, Eugene Nikolaev, Sergey Deyev

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The barnase-barstar complex is one of the most stable protein-protein complexes and has a very wide range of possible applications. Here we report the use of top-down mass spectrometry for the investigation of the structure of this complex, its ionization via ESI, isolation and fragmentation. It was found that the asymmetry of the resulting charge state distributions of the protein monomer product ions increased as the charge state of the precursor ions increased. For the investigation of the 3D structure of the complex, the gas phase H/D exchange reaction was used. In addition, supermetallized ions of the complex with Zn were produced and investigated. It was observed that an increase in the number of metals bound to the complex results in a change in complex stability and the charge distribution between protein fragment. Analysis of the fragmentation pattern of the supermetallized complex [bn-b∗+ 5Zn]10+ indicated that this ion is present in different conformations with different charges and Zn distributions. Since Zn cannot migrate, such structures must be formed during ionization.

Original languageEnglish
Article number6176
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

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Ions
Ionization
Proteins
Charge distribution
Mass spectrometry
Conformations
Monomers
Gases
Metals
Bacillus amyloliquefaciens barstar protein
Bacillus amyloliquefaciens ribonuclease

ASJC Scopus subject areas

  • General

Cite this

CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex. / Kostyukevich, Yury; Shulga, Aleksej A.; Kononikhin, Alexey; Popov, Igor; Nikolaev, Eugene; Deyev, Sergey.

In: Scientific Reports, Vol. 7, No. 1, 6176, 01.12.2017.

Research output: Contribution to journalArticle

Kostyukevich, Yury ; Shulga, Aleksej A. ; Kononikhin, Alexey ; Popov, Igor ; Nikolaev, Eugene ; Deyev, Sergey. / CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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