Chitosan-DNA complexes

Effect of molecular parameters on the efficiency of delivery

Federico Bordi, Laura Chronopoulou, Cleofe Palocci, Francesca Bomboi, Antonio Di Martino, Noemi Cifani, Barbara Pompili, Fiorentina Ascenzioni, Simona Sennato

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In the last few decades, there has been a growing interest towards the use of delivery systems for a more effective treatment of various diseases and the research increasingly focused on designing innovative solutions based on intra-cellular vectors. Among them, biocompatible DNA-polyelectrolyte complexes appear as a promising strategy for in vivo delivery of biologically active macromolecules. One of the most largely employed cationic polymer is Chitosan, which is special for its biological properties such as biodegradability, biocompatibility, mucoadhesivity, and permeability enhancer capacity. Due to this, complexes formed by condensation of DNA by Chitosan have been largely investigated for their potential use in gene therapy. Nevertheless the extensive efforts, the correlation between the physicochemical properties of the Chitosan-DNA polyplexes with their transfection efficiency still remains a central challenge. Moreover, the criteria and strategies for the design of efficient Chitosan-based gene delivery systems remain inconclusive. In a recent paper, we studied the aggregation behavior of Chitosan-DNA complexes and compared it with the predictions of existing models for the complexation of oppositely charged polyelectrolytes, showing that these models can serve as useful guide for the optimization of the complexes. Here, in order to understand the relation between physicochemical and transfection properties of Chitosan-DNA complexes, we study the efficiency of Chitosan-pDNA aggregates obtained in different conditions as vectors for DNA transfection. Small, globular and positively charged aggregates formed at large Chitosan excess, which can be obtained independently of the length of the Chitosan employed, appear to be the more effective for transfection, the more stable aggregates resulting the ones formed with longer chains.

Original languageEnglish
Pages (from-to)184-190
Number of pages7
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume460
DOIs
Publication statusPublished - 24 Dec 2014
Externally publishedYes

Fingerprint

Chitosan
delivery
DNA
deoxyribonucleic acid
gene therapy
Polyelectrolytes
biodegradability
biocompatibility
macromolecules
genes
Gene therapy
permeability
condensation
Biodegradability
Complexation
Macromolecules
Biocompatibility
optimization
Condensation
Polymers

Keywords

  • Chitosan
  • DNA
  • Gene-therapy
  • Polyelectrolytes
  • Polyplexes
  • Transfection

ASJC Scopus subject areas

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Chitosan-DNA complexes : Effect of molecular parameters on the efficiency of delivery. / Bordi, Federico; Chronopoulou, Laura; Palocci, Cleofe; Bomboi, Francesca; Di Martino, Antonio; Cifani, Noemi; Pompili, Barbara; Ascenzioni, Fiorentina; Sennato, Simona.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 460, 24.12.2014, p. 184-190.

Research output: Contribution to journalArticle

Bordi, Federico ; Chronopoulou, Laura ; Palocci, Cleofe ; Bomboi, Francesca ; Di Martino, Antonio ; Cifani, Noemi ; Pompili, Barbara ; Ascenzioni, Fiorentina ; Sennato, Simona. / Chitosan-DNA complexes : Effect of molecular parameters on the efficiency of delivery. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2014 ; Vol. 460. pp. 184-190.
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