Chemical degradation of wastes of antineoplastic agents amsacrine, azathioprine, asparaginase and thiotepa

J. Barek, J. Cvačka, J. Zima, M. De Méo, M. Laget, J. Michelon, M. Castegnaro

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

As a part of a program devoted to the destruction of antineoplastic agents, three chemical methods readily available in the hospital environment, viz. oxidation with sodium hypochlorite (NaClO, 5%), hydrogen peroxide (H2O2, 30%), and Fenton reagent (FeCl2·2H2O; 0.3 g in 10 ml H2O2, 30%), were tested for the degradation of four anticancer drugs: Amsacrine, Azathioprine, Asparaginase and Thiotepa. The efficiency of the degradation was monitored by high-performance liquid chromatography. The mutagenicity of the degradation residues mere tested by Ames test using tester strains Salmonella typhimurium TA 97a, TA 98, TA 100 and TA 102 with and without an exogenous metabolic activation system. Using sodium hypochlorite, 98.5% of Amsacrine, 99.0% of Azathioprine, 99.5% of Asparaginase and 98.7% of Thiotepa were destroyed after 1 hr. The hydrogen peroxide treatment destroyed 99% of Asparaginase and 98.7% of Thiotepa in 1 hr. However, this procedure was not efficient for the treatment of Amsacrine (28% after 16 hr) and of Azathioprine (53% degradation in 4 hr). The action of Fenton reagent resulted in the destruction of 98% of Amsacrine, and 99.5% of Azathioprine, 98.5% of Asparaginase and 98.7% of Thiotepa in 1 hr. In all cases where a high degree of degradation was achieved, the residues obtained were non mutagenic.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalAnnals of Occupational Hygiene
Volume42
Issue number4
DOIs
Publication statusPublished - 1 May 1998
Externally publishedYes

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Amsacrine
Thiotepa
Asparaginase
Azathioprine
Antineoplastic Agents
Sodium Hypochlorite
Hydrogen Peroxide
Salmonella typhimurium
High Pressure Liquid Chromatography
Pharmaceutical Preparations
Fenton's reagent

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Chemical degradation of wastes of antineoplastic agents amsacrine, azathioprine, asparaginase and thiotepa. / Barek, J.; Cvačka, J.; Zima, J.; De Méo, M.; Laget, M.; Michelon, J.; Castegnaro, M.

In: Annals of Occupational Hygiene, Vol. 42, No. 4, 01.05.1998, p. 259-266.

Research output: Contribution to journalArticle

Barek, J. ; Cvačka, J. ; Zima, J. ; De Méo, M. ; Laget, M. ; Michelon, J. ; Castegnaro, M. / Chemical degradation of wastes of antineoplastic agents amsacrine, azathioprine, asparaginase and thiotepa. In: Annals of Occupational Hygiene. 1998 ; Vol. 42, No. 4. pp. 259-266.
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