Abstract
Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)-propenyl sec-butyl disulfide (15.7-39.4%) and (Z)-propenyl sec-butyl disulfide (23.4-45.0%). Ferula essential oils stimulated [Ca2+]i mobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca2+]i flux by Ferula essential oils was dose-dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore, Ferula essential oils stimulated Ca2+ influx in TRPV1 channel-transfected HEK293 cells and desensitized the capsaicin-induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be (Z)-propenyl sec-butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.
| Original language | English |
|---|---|
| Pages (from-to) | 1361-1371 |
| Number of pages | 11 |
| Journal | Journal of Leukocyte Biology |
| Volume | 101 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jun 2017 |
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Keywords
- calcium flux
- molecular modeling
- neutrophil
- reactive oxygen species
- transient receptor potential vanilloid 1 channel
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
Chemical composition and phagocyte immunomodulatory activity of Ferula iliensis essential oils. / Özek, Gulmira; Schepetkin, Igor A.; Utegenova, Gulzhakhan A.; Kirpotina, Liliya N.; Andrei, Spencer R.; Özek, Temel; Başer, Kemal Hüsnü Can; Abidkulova, Karime T.; Kushnarenko, Svetlana V.; Khlebnikov, Andrei I.; Damron, Derek S.; Quinn, Mark T.
In: Journal of Leukocyte Biology, Vol. 101, No. 6, 01.06.2017, p. 1361-1371.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical composition and phagocyte immunomodulatory activity of Ferula iliensis essential oils
AU - Özek, Gulmira
AU - Schepetkin, Igor A.
AU - Utegenova, Gulzhakhan A.
AU - Kirpotina, Liliya N.
AU - Andrei, Spencer R.
AU - Özek, Temel
AU - Başer, Kemal Hüsnü Can
AU - Abidkulova, Karime T.
AU - Kushnarenko, Svetlana V.
AU - Khlebnikov, Andrei I.
AU - Damron, Derek S.
AU - Quinn, Mark T.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)-propenyl sec-butyl disulfide (15.7-39.4%) and (Z)-propenyl sec-butyl disulfide (23.4-45.0%). Ferula essential oils stimulated [Ca2+]i mobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca2+]i flux by Ferula essential oils was dose-dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore, Ferula essential oils stimulated Ca2+ influx in TRPV1 channel-transfected HEK293 cells and desensitized the capsaicin-induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be (Z)-propenyl sec-butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.
AB - Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)-propenyl sec-butyl disulfide (15.7-39.4%) and (Z)-propenyl sec-butyl disulfide (23.4-45.0%). Ferula essential oils stimulated [Ca2+]i mobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca2+]i flux by Ferula essential oils was dose-dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore, Ferula essential oils stimulated Ca2+ influx in TRPV1 channel-transfected HEK293 cells and desensitized the capsaicin-induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be (Z)-propenyl sec-butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.
KW - calcium flux
KW - molecular modeling
KW - neutrophil
KW - reactive oxygen species
KW - transient receptor potential vanilloid 1 channel
UR - http://www.scopus.com/inward/record.url?scp=85029667938&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029667938&partnerID=8YFLogxK
U2 - 10.1189/jlb.3A1216-518RR
DO - 10.1189/jlb.3A1216-518RR
M3 - Article
VL - 101
SP - 1361
EP - 1371
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 6
ER -