Characterization of distinct macrophage subpopulations during nitrogen mustard-induced lung injury and fibrosis

Alessandro Venosa, Rama Malaviya, Hyejeong Choi, Andrew J. Gow, Jeffrey D. Laskin, Debra L. Laskin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Nitrogen mustard (NM) is an alkylating agent known to cause extensive pulmonary injury progressing to fibrosis. This is accompanied by a persistent macrophage inflammatory response. In these studies, we characterized the phenotype of macrophages accumulating in the lung over time following NM exposure. Treatment of rats withNM(0.125 mg/kg, intratracheally) resulted in an increase in CD11b+ macrophages in histologic sections. These cells consisted of inducible nitric oxide synthase1 (iNOS) proinflammatory M1 macrophages, and CD68+, CD163+, CD206+, YM-1+, and arginase-II+ antiinflammatory M2 macrophages. Although M1 macrophages were prominent 1-3 days after NM, M2 macrophages were most notable at 28 days. At this time, they were enlarged and vacuolated, consistent with a profibrotic phenotype. Flow cytometric analysis of isolated lung macrophages identified three phenotypically distinct subpopulations: mature CD11b-, CD43-, and CD68-, resident macrophages, which decreased in numbers after NM; and two infiltrating (CD11b+,) macrophage subsets: immature CD431 M+, macrophages and mature CD43-, M2 macrophages, which increased sequentially. Time-related increases in M1 (iNOS, IL-12α, COX-2, TNF-α, matrix metalloproteinase-9, matrix metalloproteinase-10) and M2 (IL-10, pentraxin-2, connective tissue growth factor, ApoE) genes, as well as chemokines/ chemokine receptors associated with trafficking ofM1(CCR2, CCR5, CCL2, CCL5) and M2 (CX3CR1, fractalkine) macrophages to sites of injury, were also noted in macrophages isolated from the lung after NM. The appearance of M1 and M2 macrophages in the lung correlated with NM-induced acute injury and the development of fibrosis, suggesting a potential role of these macrophage subpopulations in the pathogenic response to NM.

Original languageEnglish
Pages (from-to)436-446
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume54
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

Fingerprint

Mechlorethamine
Macrophages
Lung Injury
Fibrosis
Lung
Nitric Oxide
Matrix Metalloproteinase 10
Chemokine CX3CL1
Connective Tissue Growth Factor
Phenotype
Arginase
Chemokine Receptors
Alkylating Agents
Matrix Metalloproteinase 9
Wounds and Injuries
Apolipoproteins E
Interleukin-12

Keywords

  • Fibrosis
  • Inflammation
  • Macrophages
  • Nitrogen mustard

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Characterization of distinct macrophage subpopulations during nitrogen mustard-induced lung injury and fibrosis. / Venosa, Alessandro; Malaviya, Rama; Choi, Hyejeong; Gow, Andrew J.; Laskin, Jeffrey D.; Laskin, Debra L.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 54, No. 3, 01.03.2016, p. 436-446.

Research output: Contribution to journalArticle

Venosa, Alessandro ; Malaviya, Rama ; Choi, Hyejeong ; Gow, Andrew J. ; Laskin, Jeffrey D. ; Laskin, Debra L. / Characterization of distinct macrophage subpopulations during nitrogen mustard-induced lung injury and fibrosis. In: American Journal of Respiratory Cell and Molecular Biology. 2016 ; Vol. 54, No. 3. pp. 436-446.
@article{df0de71886c444ac98c34bf31526326c,
title = "Characterization of distinct macrophage subpopulations during nitrogen mustard-induced lung injury and fibrosis",
abstract = "Nitrogen mustard (NM) is an alkylating agent known to cause extensive pulmonary injury progressing to fibrosis. This is accompanied by a persistent macrophage inflammatory response. In these studies, we characterized the phenotype of macrophages accumulating in the lung over time following NM exposure. Treatment of rats withNM(0.125 mg/kg, intratracheally) resulted in an increase in CD11b+ macrophages in histologic sections. These cells consisted of inducible nitric oxide synthase1 (iNOS) proinflammatory M1 macrophages, and CD68+, CD163+, CD206+, YM-1+, and arginase-II+ antiinflammatory M2 macrophages. Although M1 macrophages were prominent 1-3 days after NM, M2 macrophages were most notable at 28 days. At this time, they were enlarged and vacuolated, consistent with a profibrotic phenotype. Flow cytometric analysis of isolated lung macrophages identified three phenotypically distinct subpopulations: mature CD11b-, CD43-, and CD68-, resident macrophages, which decreased in numbers after NM; and two infiltrating (CD11b+,) macrophage subsets: immature CD431 M+, macrophages and mature CD43-, M2 macrophages, which increased sequentially. Time-related increases in M1 (iNOS, IL-12α, COX-2, TNF-α, matrix metalloproteinase-9, matrix metalloproteinase-10) and M2 (IL-10, pentraxin-2, connective tissue growth factor, ApoE) genes, as well as chemokines/ chemokine receptors associated with trafficking ofM1(CCR2, CCR5, CCL2, CCL5) and M2 (CX3CR1, fractalkine) macrophages to sites of injury, were also noted in macrophages isolated from the lung after NM. The appearance of M1 and M2 macrophages in the lung correlated with NM-induced acute injury and the development of fibrosis, suggesting a potential role of these macrophage subpopulations in the pathogenic response to NM.",
keywords = "Fibrosis, Inflammation, Macrophages, Nitrogen mustard",
author = "Alessandro Venosa and Rama Malaviya and Hyejeong Choi and Gow, {Andrew J.} and Laskin, {Jeffrey D.} and Laskin, {Debra L.}",
year = "2016",
month = "3",
day = "1",
doi = "10.1165/rcmb.2015-0120OC",
language = "English",
volume = "54",
pages = "436--446",
journal = "American Journal of Respiratory Cell and Molecular Biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "3",

}

TY - JOUR

T1 - Characterization of distinct macrophage subpopulations during nitrogen mustard-induced lung injury and fibrosis

AU - Venosa, Alessandro

AU - Malaviya, Rama

AU - Choi, Hyejeong

AU - Gow, Andrew J.

AU - Laskin, Jeffrey D.

AU - Laskin, Debra L.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Nitrogen mustard (NM) is an alkylating agent known to cause extensive pulmonary injury progressing to fibrosis. This is accompanied by a persistent macrophage inflammatory response. In these studies, we characterized the phenotype of macrophages accumulating in the lung over time following NM exposure. Treatment of rats withNM(0.125 mg/kg, intratracheally) resulted in an increase in CD11b+ macrophages in histologic sections. These cells consisted of inducible nitric oxide synthase1 (iNOS) proinflammatory M1 macrophages, and CD68+, CD163+, CD206+, YM-1+, and arginase-II+ antiinflammatory M2 macrophages. Although M1 macrophages were prominent 1-3 days after NM, M2 macrophages were most notable at 28 days. At this time, they were enlarged and vacuolated, consistent with a profibrotic phenotype. Flow cytometric analysis of isolated lung macrophages identified three phenotypically distinct subpopulations: mature CD11b-, CD43-, and CD68-, resident macrophages, which decreased in numbers after NM; and two infiltrating (CD11b+,) macrophage subsets: immature CD431 M+, macrophages and mature CD43-, M2 macrophages, which increased sequentially. Time-related increases in M1 (iNOS, IL-12α, COX-2, TNF-α, matrix metalloproteinase-9, matrix metalloproteinase-10) and M2 (IL-10, pentraxin-2, connective tissue growth factor, ApoE) genes, as well as chemokines/ chemokine receptors associated with trafficking ofM1(CCR2, CCR5, CCL2, CCL5) and M2 (CX3CR1, fractalkine) macrophages to sites of injury, were also noted in macrophages isolated from the lung after NM. The appearance of M1 and M2 macrophages in the lung correlated with NM-induced acute injury and the development of fibrosis, suggesting a potential role of these macrophage subpopulations in the pathogenic response to NM.

AB - Nitrogen mustard (NM) is an alkylating agent known to cause extensive pulmonary injury progressing to fibrosis. This is accompanied by a persistent macrophage inflammatory response. In these studies, we characterized the phenotype of macrophages accumulating in the lung over time following NM exposure. Treatment of rats withNM(0.125 mg/kg, intratracheally) resulted in an increase in CD11b+ macrophages in histologic sections. These cells consisted of inducible nitric oxide synthase1 (iNOS) proinflammatory M1 macrophages, and CD68+, CD163+, CD206+, YM-1+, and arginase-II+ antiinflammatory M2 macrophages. Although M1 macrophages were prominent 1-3 days after NM, M2 macrophages were most notable at 28 days. At this time, they were enlarged and vacuolated, consistent with a profibrotic phenotype. Flow cytometric analysis of isolated lung macrophages identified three phenotypically distinct subpopulations: mature CD11b-, CD43-, and CD68-, resident macrophages, which decreased in numbers after NM; and two infiltrating (CD11b+,) macrophage subsets: immature CD431 M+, macrophages and mature CD43-, M2 macrophages, which increased sequentially. Time-related increases in M1 (iNOS, IL-12α, COX-2, TNF-α, matrix metalloproteinase-9, matrix metalloproteinase-10) and M2 (IL-10, pentraxin-2, connective tissue growth factor, ApoE) genes, as well as chemokines/ chemokine receptors associated with trafficking ofM1(CCR2, CCR5, CCL2, CCL5) and M2 (CX3CR1, fractalkine) macrophages to sites of injury, were also noted in macrophages isolated from the lung after NM. The appearance of M1 and M2 macrophages in the lung correlated with NM-induced acute injury and the development of fibrosis, suggesting a potential role of these macrophage subpopulations in the pathogenic response to NM.

KW - Fibrosis

KW - Inflammation

KW - Macrophages

KW - Nitrogen mustard

UR - http://www.scopus.com/inward/record.url?scp=84963900850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84963900850&partnerID=8YFLogxK

U2 - 10.1165/rcmb.2015-0120OC

DO - 10.1165/rcmb.2015-0120OC

M3 - Article

VL - 54

SP - 436

EP - 446

JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

IS - 3

ER -