Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration

Abstract

A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.

Original language	English
Pages (from-to)	67-77
Number of pages	11
Journal	European Polymer Journal
Volume	113
DOIs	https://doi.org/10.1016/j.eurpolymj.2019.01.042
Publication status	Published - 1 Apr 2019

Keywords

Electrospinning
Scaffold
Silicate-containing hydroxyapatite
Strontium-containing hydroxyapatite

ASJC Scopus subject areas

Physics and Astronomy(all)
Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.eurpolymj.2019.01.042

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Surmenev, R. A., Shkarina, S., Syromotina, D. S., Melnik, E. V., Shkarin, R., Selezneva, I. I., Ermakov, A. M., Ivlev, S. I., Cecilia, A., Weinhardt, V., Baumbach, T., Rijavec, T., Lapanje, A., Chaikina, M. V., & Surmeneva, M. A. (2019). Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration. European Polymer Journal, 113, 67-77. https://doi.org/10.1016/j.eurpolymj.2019.01.042

Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration. / Surmenev, Roman A.; Shkarina, Svetlana; Syromotina, Dina S.; Melnik, Elizaveta V.; Shkarin, Roman; Selezneva, Irina I.; Ermakov, Artem M.; Ivlev, Sergei I.; Cecilia, Angelica; Weinhardt, Venera; Baumbach, Tilo; Rijavec, Tomaž; Lapanje, Ales; Chaikina, Marina V.; Surmeneva, Maria A.

In: European Polymer Journal, Vol. 113, 01.04.2019, p. 67-77.

Research output: Contribution to journal › Article

Surmenev, RA , Shkarina, S, Syromotina, DS, Melnik, EV, Shkarin, R, Selezneva, II, Ermakov, AM, Ivlev, SI, Cecilia, A, Weinhardt, V, Baumbach, T, Rijavec, T, Lapanje, A, Chaikina, MV & Surmeneva, MA 2019, 'Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration', European Polymer Journal, vol. 113, pp. 67-77. https://doi.org/10.1016/j.eurpolymj.2019.01.042

Surmenev, Roman A. ; Shkarina, Svetlana ; Syromotina, Dina S. ; Melnik, Elizaveta V. ; Shkarin, Roman ; Selezneva, Irina I. ; Ermakov, Artem M. ; Ivlev, Sergei I. ; Cecilia, Angelica ; Weinhardt, Venera ; Baumbach, Tilo ; Rijavec, Tomaž ; Lapanje, Ales ; Chaikina, Marina V. ; Surmeneva, Maria A. / Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration. In: European Polymer Journal. 2019 ; Vol. 113. pp. 67-77.

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abstract = "A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.",

keywords = "Electrospinning, Scaffold, Silicate-containing hydroxyapatite, Strontium-containing hydroxyapatite",

author = "Surmenev, {Roman A.} and Svetlana Shkarina and Syromotina, {Dina S.} and Melnik, {Elizaveta V.} and Roman Shkarin and Selezneva, {Irina I.} and Ermakov, {Artem M.} and Ivlev, {Sergei I.} and Angelica Cecilia and Venera Weinhardt and Tilo Baumbach and Toma{\v z} Rijavec and Ales Lapanje and Chaikina, {Marina V.} and Surmeneva, {Maria A.}",

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AU - Surmenev, Roman A.

AU - Shkarina, Svetlana

AU - Syromotina, Dina S.

AU - Melnik, Elizaveta V.

AU - Shkarin, Roman

AU - Selezneva, Irina I.

AU - Ermakov, Artem M.

AU - Ivlev, Sergei I.

AU - Cecilia, Angelica

AU - Weinhardt, Venera

AU - Baumbach, Tilo

AU - Rijavec, Tomaž

AU - Lapanje, Ales

AU - Chaikina, Marina V.

AU - Surmeneva, Maria A.

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N2 - A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.

AB - A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.

KW - Electrospinning

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KW - Strontium-containing hydroxyapatite

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