Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration

Roman A. Surmenev, Svetlana Shkarina, Dina S. Syromotina, Elizaveta V. Melnik, Roman Shkarin, Irina I. Selezneva, Artem M. Ermakov, Sergei I. Ivlev, Angelica Cecilia, Venera Weinhardt, Tilo Baumbach, Tomaž Rijavec, Ales Lapanje, Marina V. Chaikina, Maria A. Surmeneva

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.

Original languageEnglish
Pages (from-to)67-77
Number of pages11
JournalEuropean Polymer Journal
Volume113
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • Electrospinning
  • Scaffold
  • Silicate-containing hydroxyapatite
  • Strontium-containing hydroxyapatite

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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    Surmenev, R. A., Shkarina, S., Syromotina, D. S., Melnik, E. V., Shkarin, R., Selezneva, I. I., Ermakov, A. M., Ivlev, S. I., Cecilia, A., Weinhardt, V., Baumbach, T., Rijavec, T., Lapanje, A., Chaikina, M. V., & Surmeneva, M. A. (2019). Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration. European Polymer Journal, 113, 67-77. https://doi.org/10.1016/j.eurpolymj.2019.01.042