Abstract
A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.
| Original language | English |
|---|---|
| Pages (from-to) | 67-77 |
| Number of pages | 11 |
| Journal | European Polymer Journal |
| Volume | 113 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
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Keywords
- Electrospinning
- Scaffold
- Silicate-containing hydroxyapatite
- Strontium-containing hydroxyapatite
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry
Cite this
Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration. / Surmenev, Roman A.; Shkarina, Svetlana; Syromotina, Dina S.; Melnik, Elizaveta V.; Shkarin, Roman; Selezneva, Irina I.; Ermakov, Artem M.; Ivlev, Sergei I.; Cecilia, Angelica; Weinhardt, Venera; Baumbach, Tilo; Rijavec, Tomaž; Lapanje, Ales; Chaikina, Marina V.; Surmeneva, Maria A.
In: European Polymer Journal, Vol. 113, 01.04.2019, p. 67-77.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration
AU - Surmenev, Roman A.
AU - Shkarina, Svetlana
AU - Syromotina, Dina S.
AU - Melnik, Elizaveta V.
AU - Shkarin, Roman
AU - Selezneva, Irina I.
AU - Ermakov, Artem M.
AU - Ivlev, Sergei I.
AU - Cecilia, Angelica
AU - Weinhardt, Venera
AU - Baumbach, Tilo
AU - Rijavec, Tomaž
AU - Lapanje, Ales
AU - Chaikina, Marina V.
AU - Surmeneva, Maria A.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.
AB - A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.
KW - Electrospinning
KW - Scaffold
KW - Silicate-containing hydroxyapatite
KW - Strontium-containing hydroxyapatite
UR - http://www.scopus.com/inward/record.url?scp=85060526981&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060526981&partnerID=8YFLogxK
U2 - 10.1016/j.eurpolymj.2019.01.042
DO - 10.1016/j.eurpolymj.2019.01.042
M3 - Article
VL - 113
SP - 67
EP - 77
JO - European Polymer Journal
JF - European Polymer Journal
SN - 0014-3057
ER -