Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone

Matthias Wiens, Tarek A. Elkhooly, Heinz Christoph Schröder, Tawheed H.A. Mohamed, Werner E.G. Müller

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering. However, such composites generally have no osteogenic activity per se. Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability. Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed. Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity. These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.

Original languageEnglish
Pages (from-to)4456-4464
Number of pages9
JournalActa Biomaterialia
Volume10
Issue number10
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Fingerprint

Polycaprolactone
Chitosan
Grafts
Transplants
Bone
Composite materials
Wettability
Bone and Bones
Immobilized Enzymes
Bone Regeneration
Polyesters
Hybrid materials
Phosphatases
Tissue Engineering
Polysaccharides
Tissue engineering
Adsorption
Surface morphology
Minerals
Alkaline Phosphatase

Keywords

  • Biosilica
  • Chitosan
  • Osteogenic activity
  • Polycaprolactone
  • Silicatein

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone. / Wiens, Matthias; Elkhooly, Tarek A.; Schröder, Heinz Christoph; Mohamed, Tawheed H.A.; Müller, Werner E.G.

In: Acta Biomaterialia, Vol. 10, No. 10, 01.01.2014, p. 4456-4464.

Research output: Contribution to journalArticle

Wiens, Matthias ; Elkhooly, Tarek A. ; Schröder, Heinz Christoph ; Mohamed, Tawheed H.A. ; Müller, Werner E.G. / Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 10. pp. 4456-4464.
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