Kharakteristika deǐstviia Ca/Mg-zavisimoǐ éndonukleazy kletochnykh iader limfotsitov cheloveka na J kappa-klaster immunoglobulinovykh genov.

Translated title of the contribution: Characteristics of the effect of Ca/Mg-dependent endonuclease from cell nuclei of human lymphocytes on J kappa-cluster of immunoglobulin genes

A. I. Morozov, D. N. Urakov, N. N. Khodarev, S. M. Deev, O. L. Polianovskiǐ

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1 Citation (Scopus)

Abstract

The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.

Translated title of the contributionCharacteristics of the effect of Ca/Mg-dependent endonuclease from cell nuclei of human lymphocytes on J kappa-cluster of immunoglobulin genes
Original languageRussian
Pages (from-to)26-29
Number of pages4
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number11
Publication statusPublished - Nov 1988

ASJC Scopus subject areas

  • Medicine(all)

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