Kharakteristika deǐstviia Ca/Mg-zavisimoǐ éndonukleazy kletochnykh iader limfotsitov cheloveka na J kappa-klaster immunoglobulinovykh genov.

Translated title of the contribution: Characteristics of the effect of Ca/Mg-dependent endonuclease from cell nuclei of human lymphocytes on J kappa-cluster of immunoglobulin genes

A. I. Morozov, D. N. Urakov, N. N. Khodarev, S. M. Deev, O. L. Polianovskiǐ

Research Nuclear Reactor Center

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.

Translated title of the contribution	Characteristics of the effect of Ca/Mg-dependent endonuclease from cell nuclei of human lymphocytes on J kappa-cluster of immunoglobulin genes
Original language	Russian
Pages (from-to)	26-29
Number of pages	4
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	11
Publication status	Published - Nov 1988

ASJC Scopus subject areas

Medicine(all)

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Kharakteristika deǐstviia Ca/Mg-zavisimoǐ éndonukleazy kletochnykh iader limfotsitov cheloveka na J kappa-klaster immunoglobulinovykh genov. / Morozov, A. I.; Urakov, D. N.; Khodarev, N. N.; Deev, S. M.; Polianovskiǐ, O. L.

In: Molekuliarnaia genetika, mikrobiologiia i virusologiia, No. 11, 11.1988, p. 26-29.

Research output: Contribution to journal › Article › peer-review

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title = "Kharakteristika deǐstviia Ca/Mg-zavisimoǐ {\'e}ndonukleazy kletochnykh iader limfotsitov cheloveka na J kappa-klaster immunoglobulinovykh genov.",

abstract = "The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.",

author = "Morozov, {A. I.} and Urakov, {D. N.} and Khodarev, {N. N.} and Deev, {S. M.} and Polianovskiǐ, {O. L.}",

year = "1988",

month = nov,

language = "Русский",

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T1 - Kharakteristika deǐstviia Ca/Mg-zavisimoǐ éndonukleazy kletochnykh iader limfotsitov cheloveka na J kappa-klaster immunoglobulinovykh genov.

AU - Morozov, A. I.

AU - Urakov, D. N.

AU - Khodarev, N. N.

AU - Deev, S. M.

AU - Polianovskiǐ, O. L.

PY - 1988/11

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N2 - The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.

AB - The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.

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