Cellular processing of 125I- and 111In-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells

Anna Orlova, Alexander Bruskin, Anna Sjöström, Hans Lundqvist, Lars Gedda, Vladimir Tolmachev

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.

Original languageEnglish
Pages (from-to)827-835
Number of pages9
JournalNuclear Medicine and Biology
Volume27
Issue number8
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • In-DTPA
  • I-para-iodo-benzoate
  • A431 cells
  • EGF
  • Intracellular retention

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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