Cellular processing of 125I- and 111In-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells

Abstract

Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.

Original language	English
Pages (from-to)	827-835
Number of pages	9
Journal	Nuclear Medicine and Biology
Volume	27
Issue number	8
DOIs	https://doi.org/10.1016/S0969-8051(00)00148-7
Publication status	Published - 2000
Externally published	Yes

Keywords

In-DTPA
I-para-iodo-benzoate
A431 cells
EGF
Intracellular retention

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Cancer Research

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10.1016/S0969-8051(00)00148-7

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title = "Cellular processing of 125I- and 111In-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells",

abstract = "Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.",

keywords = "In-DTPA, I-para-iodo-benzoate, A431 cells, EGF, Intracellular retention",

author = "Anna Orlova and Alexander Bruskin and Anna Sj{\"o}str{\"o}m and Hans Lundqvist and Lars Gedda and Vladimir Tolmachev",

year = "2000",

doi = "10.1016/S0969-8051(00)00148-7",

language = "English",

volume = "27",

pages = "827--835",

journal = "Nuclear Medicine and Biology",

issn = "0969-8051",

publisher = "Elsevier Inc.",

number = "8",

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TY - JOUR

T1 - Cellular processing of 125I- and 111In-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells

AU - Orlova, Anna

AU - Bruskin, Alexander

AU - Sjöström, Anna

AU - Lundqvist, Hans

AU - Gedda, Lars

AU - Tolmachev, Vladimir

PY - 2000

Y1 - 2000

N2 - Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.

AB - Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.

KW - In-DTPA

KW - I-para-iodo-benzoate

KW - A431 cells

KW - EGF

KW - Intracellular retention

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Cellular processing of ¹²⁵I- and ¹¹¹In-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint Dive into the research topics of 'Cellular processing of <sup>125</sup>I- and <sup>111</sup>In-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells'. Together they form a unique fingerprint.

Cite this