Cellular processing in the SW1222 cell line of mAb A33 directly and indirectly radiohalogenated

Johanna Höglund, Anna Orlova, Anders Sundin, Hans Lundqvist, Vladimir Tolmachev

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Investigations into the cellular processing of radio-labeled monoclonal antibodies (mAbs) for their further use in radioimmunodiagnosis and cancer therapy are needed in order to understand the fate of internalized and catabolized mAbs. The anti-colorectal cancer mAb, A33, was labelled with 76Br and 125I using the direct Chloramine-T method, or by labelling N-succinimidyl para-(tri-methylstannyl) benzoate and its further conjugation to the mAb. The cellular processing of the four conjugates was investigated in SW1222 cells in vitro. Uptake of mAb was rapid, peaking after 14-16 h. Intracellular degradation was slow and the early loss of radioactivity was due to dissociation of cell-surface bound mAb. The indirect labelling resulted in stronger binding of the mAb as well as prolonged intracellular retention of the radiolabel. Direct and indirect halogen radiolabelling results in different cellprocessing patterns of radiolabels, and radioactive catabolic products follow different routes of cellular excretion. The results of this cellular study indicate that indirect labelling is preferable to the direct Chloramine-T method.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalOncology Reports
Volume16
Issue number1
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Keywords

  • Antibody internalization
  • Cellular processing
  • Indirect labeling

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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