Cell-IQ visualization of motility, cell mass, and osteogenic differentiation of multipotent mesenchymal stromal cells cultured with relief calcium phosphate coating

L. S. Litvinova, V. V. Shupletsova, K. A. Yurova, O. G. Khaziakhmatova, N. M. Todosenko, M. Yu Khlusova, G. B. Slepchenko, E. G. Cherempey, Yu P. Sharkeev, E. G. Komarova, M. B. Sedelnikova, V. V. Malashchenko, E. S. Melashchenko, I. A. Khlusov

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The Cell-IQ continuous surveillance system allowed us to establish the following changes in a 14- day culture in vitro: a twofold suppression of the directional migration of multipotent mesenchymal stromal cells of human adipose tissue (MMSC-AT) towards the samples with a microarc calcium phosphate (CP) coating from synthetic hydroxyapatite; a tenfold decrease in the cell mass on the interphase with the samples, which was accompanied by a slight reduction in the expression of membrane determinants of stromal stem cells; and an enhancement of their osteogenic differentiation (osteocalcin secretion and mineralized matrix formation) on the 21st day of the study. Calcium phosphate particles, but not the calcium and phosphorus ions, may trigger the phenotypic transformation of the MMSC-AT behavior in vitro.

Original languageEnglish
Pages (from-to)310-315
Number of pages6
JournalDoklady Biochemistry and Biophysics
Volume476
Issue number1
DOIs
Publication statusPublished - 1 Sep 2017

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Chemistry(all)

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