Abstract
Irreversible cardiomyocyte damage was induced by 45-min global ischemia followed by 30- min reperfusion in Langendorff-perfused isolated rat heart. Cell damage was assessed by the release of creatine phosphokinase into the perfusate. The hearts were subjected to the following postconditioning protocols: 1) three cycles of 10-sec reperfusion and 10-sec ischemia, total cycle time 20 sec; 2) six cycles of 10-sec reperfusion and 10-sec ischemia, total cycle time 20 sec; 3) three sessions of 20-sec reperfusion and 20-sec ischemia, total cycle time 40 sec; 4) 6 cycles of 20-sec reperfusion and 20-sec ischemia, total cycle time 40 sec; 5) 3 cycles of 30-sec reperfusion and 30-sec ischemia, total cycle time 60 sec. It was found that only postconditioning with a total cycle time of 40 sec or 60 sec prevents myocardial reperfusion injury.
Original language | English |
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Pages (from-to) | 313-314 |
Number of pages | 2 |
Journal | Bulletin of Experimental Biology and Medicine |
Volume | 153 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jul 2012 |
Keywords
- Heart
- Ischemia
- Postconditioning
- Reperfusion
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)