Cancer gene therapy with plasmid expressing anti-HER2/neu-Toxin

Abstract

The HER2/neu receptor is over-expressed on the surface of many types of cancer cells, and is widely used for targeted delivery of anticancer drugs. We have created genetically engineered construct expressing the PE40 fragment of Pseudomonas toxin bound with the DARPin molecule which recognizes the HER2/neu receptor with high specificity. The construct destroyed transfected tumor cells in vitro. Intra-Tumor injections of the construct complexed with polyethyleneimine led to growth retardation of D2F2/E2 tumors in mice. These results suggest a possibility of using this approach to develop new anticancer drugs.

Original language	English
Pages (from-to)	671-675
Number of pages	5
Journal	Voprosy Onkologii
Volume	62
Issue number	5
Publication status	Published - 2016
Externally published	Yes

Keywords

Darpin
Gene therapy
HER2/NEU receptor
Pseudomonas exotoxin a
Targeted therapy

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Cancer gene therapy with plasmid expressing anti-HER2/neu-Toxin",

abstract = "The HER2/neu receptor is over-expressed on the surface of many types of cancer cells, and is widely used for targeted delivery of anticancer drugs. We have created genetically engineered construct expressing the PE40 fragment of Pseudomonas toxin bound with the DARPin molecule which recognizes the HER2/neu receptor with high specificity. The construct destroyed transfected tumor cells in vitro. Intra-Tumor injections of the construct complexed with polyethyleneimine led to growth retardation of D2F2/E2 tumors in mice. These results suggest a possibility of using this approach to develop new anticancer drugs.",

keywords = "Darpin, Gene therapy, HER2/NEU receptor, Pseudomonas exotoxin a, Targeted therapy",

author = "Khodarovich, {Yu M.} and Rakhmaninova, {D. D.} and Kagarlitskiy, {G. O.} and Deev, {S. M.}",

year = "2016",

language = "English",

volume = "62",

pages = "671--675",

journal = "Voprosy Onkologii",

issn = "0507-3758",

publisher = "Izdatelstvo Meditsina",

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T1 - Cancer gene therapy with plasmid expressing anti-HER2/neu-Toxin

AU - Khodarovich, Yu M.

AU - Rakhmaninova, D. D.

AU - Kagarlitskiy, G. O.

AU - Deev, S. M.

PY - 2016

Y1 - 2016

N2 - The HER2/neu receptor is over-expressed on the surface of many types of cancer cells, and is widely used for targeted delivery of anticancer drugs. We have created genetically engineered construct expressing the PE40 fragment of Pseudomonas toxin bound with the DARPin molecule which recognizes the HER2/neu receptor with high specificity. The construct destroyed transfected tumor cells in vitro. Intra-Tumor injections of the construct complexed with polyethyleneimine led to growth retardation of D2F2/E2 tumors in mice. These results suggest a possibility of using this approach to develop new anticancer drugs.

AB - The HER2/neu receptor is over-expressed on the surface of many types of cancer cells, and is widely used for targeted delivery of anticancer drugs. We have created genetically engineered construct expressing the PE40 fragment of Pseudomonas toxin bound with the DARPin molecule which recognizes the HER2/neu receptor with high specificity. The construct destroyed transfected tumor cells in vitro. Intra-Tumor injections of the construct complexed with polyethyleneimine led to growth retardation of D2F2/E2 tumors in mice. These results suggest a possibility of using this approach to develop new anticancer drugs.

KW - Darpin

KW - Gene therapy

KW - HER2/NEU receptor

KW - Pseudomonas exotoxin a

KW - Targeted therapy

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