Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells

V. V. Malashchenko, E. O. Shunkin, V. V. Shupletsova, O. G. Khaziakhmatova, K. A. Yurova, E. S. Melashchenko, L. S. Litvinova, I. A. Khlusov, E. G. Komarova, V. V. Chebodaeva, Yu P. Sharkeev

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Modern biomaterial biocompatibility research focuses the biomaterial hierarchic effects on multipotent mesenchymal stromal cell (MMSC) behavior (that occur at the nano-, micro- and macroscales) because MMSCs are the fundamental units that produce/regenerate bone tissue. Leukemia initiation and progression are connected with a disfunction of health cell microenvironment and MMSCs. Continuous monitoring of MMSC and tumor cell interaction is a promising tool for oncology, cellular biology, biotechnology and environmental research. The aim was to investigate a modulation of in vitro interaction of human MMSCs and leukemic T lymphoblast-like cells (Jurkat T cells) caused by micro-arc multilevel calcium phosphate (CP) coating with the help of Cell-IQ and RTCA advanced tools for continuous monitoring. An average velocity of cell division (AVCD) of human adipose-derived MMSCs (hAMMSCs) contacted in vitro with allogenic Jurkat line of human leukemic T lymphoblasts (Jurkat T cells) was studied by means of Cell-IQ v2 MLF integrated phase-contrast microscopic platform for real-time surveillance imaging of living cells. Both 50 μL suspensions (5×104 viable karyocytes) of the CD73CD90CD105+ adherent cells and Jurkat T cells were applied into the center of the well of 12-well plastic plates for 7 days at 100% humidity in a 5% CO2 atmosphere at 37°C until a monolayer formation. A nutrient medium was once replaced. To determine cell invasion (chemotactic motility) through 8 μm pores the real-time cell analysis (RTCA DP Analyzer) with the CIM-plate was used. AVCD of fibroblast-like adherent hAMMSCs was 0.27-0.63 divisions/h. CP coating diminished significantly the percent of dividing hAMMSCs contacted with leukemic Jurkat T cells. RTCA system showed significant hAMMSC invasion towards tumor cells and not vice versa. For all this, cellular interaction led to increasing viability of Jurkat T cells and decreasing hAMMSC viability. Thus, tumor Jurkat T cells could control a fate of health hAMMSCs and promote stromal microenvironment for survivability of tumor clones by means of secretable molecular products. Multilevel micro-arc CP coating forms bone-like inorganic structure that is capable to modulate in vitro interaction of human MMSCs and leukemic T lymphoblasts. The results obtained may be useful for replacement surgery applications of orthopedic implants in cancer patients.

Original languageEnglish
Title of host publicationProceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019
EditorsVictor E. Panin, Sergey G. Psakhie, Vasily M. Fomin
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419124
DOIs
Publication statusPublished - 19 Nov 2019
EventInternational Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 - Tomsk, Russian Federation
Duration: 1 Oct 20195 Oct 2019

Publication series

NameAIP Conference Proceedings
Volume2167
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

ConferenceInternational Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019
CountryRussian Federation
CityTomsk
Period1.10.195.10.19

Fingerprint

calcium phosphates
stem cells
bones
tumors
coatings
interactions
cells
cell division
viability
health
arcs
plastic plates
orthopedics
biotechnology
leukemias
locomotion
fibroblasts
phase contrast
nutrients
biocompatibility

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Malashchenko, V. V., Shunkin, E. O., Shupletsova, V. V., Khaziakhmatova, O. G., Yurova, K. A., Melashchenko, E. S., ... Sharkeev, Y. P. (2019). Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells. In V. E. Panin, S. G. Psakhie, & V. M. Fomin (Eds.), Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 [020215] (AIP Conference Proceedings; Vol. 2167). American Institute of Physics Inc.. https://doi.org/10.1063/1.5132082

Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells. / Malashchenko, V. V.; Shunkin, E. O.; Shupletsova, V. V.; Khaziakhmatova, O. G.; Yurova, K. A.; Melashchenko, E. S.; Litvinova, L. S.; Khlusov, I. A.; Komarova, E. G.; Chebodaeva, V. V.; Sharkeev, Yu P.

Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019. ed. / Victor E. Panin; Sergey G. Psakhie; Vasily M. Fomin. American Institute of Physics Inc., 2019. 020215 (AIP Conference Proceedings; Vol. 2167).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Malashchenko, VV, Shunkin, EO, Shupletsova, VV, Khaziakhmatova, OG, Yurova, KA, Melashchenko, ES, Litvinova, LS, Khlusov, IA, Komarova, EG, Chebodaeva, VV & Sharkeev, YP 2019, Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells. in VE Panin, SG Psakhie & VM Fomin (eds), Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019., 020215, AIP Conference Proceedings, vol. 2167, American Institute of Physics Inc., International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019, Tomsk, Russian Federation, 1.10.19. https://doi.org/10.1063/1.5132082
Malashchenko VV, Shunkin EO, Shupletsova VV, Khaziakhmatova OG, Yurova KA, Melashchenko ES et al. Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells. In Panin VE, Psakhie SG, Fomin VM, editors, Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019. American Institute of Physics Inc. 2019. 020215. (AIP Conference Proceedings). https://doi.org/10.1063/1.5132082
Malashchenko, V. V. ; Shunkin, E. O. ; Shupletsova, V. V. ; Khaziakhmatova, O. G. ; Yurova, K. A. ; Melashchenko, E. S. ; Litvinova, L. S. ; Khlusov, I. A. ; Komarova, E. G. ; Chebodaeva, V. V. ; Sharkeev, Yu P. / Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells. Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019. editor / Victor E. Panin ; Sergey G. Psakhie ; Vasily M. Fomin. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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abstract = "Modern biomaterial biocompatibility research focuses the biomaterial hierarchic effects on multipotent mesenchymal stromal cell (MMSC) behavior (that occur at the nano-, micro- and macroscales) because MMSCs are the fundamental units that produce/regenerate bone tissue. Leukemia initiation and progression are connected with a disfunction of health cell microenvironment and MMSCs. Continuous monitoring of MMSC and tumor cell interaction is a promising tool for oncology, cellular biology, biotechnology and environmental research. The aim was to investigate a modulation of in vitro interaction of human MMSCs and leukemic T lymphoblast-like cells (Jurkat T cells) caused by micro-arc multilevel calcium phosphate (CP) coating with the help of Cell-IQ and RTCA advanced tools for continuous monitoring. An average velocity of cell division (AVCD) of human adipose-derived MMSCs (hAMMSCs) contacted in vitro with allogenic Jurkat line of human leukemic T lymphoblasts (Jurkat T cells) was studied by means of Cell-IQ v2 MLF integrated phase-contrast microscopic platform for real-time surveillance imaging of living cells. Both 50 μL suspensions (5×104 viable karyocytes) of the CD73CD90CD105+ adherent cells and Jurkat T cells were applied into the center of the well of 12-well plastic plates for 7 days at 100{\%} humidity in a 5{\%} CO2 atmosphere at 37°C until a monolayer formation. A nutrient medium was once replaced. To determine cell invasion (chemotactic motility) through 8 μm pores the real-time cell analysis (RTCA DP Analyzer) with the CIM-plate was used. AVCD of fibroblast-like adherent hAMMSCs was 0.27-0.63 divisions/h. CP coating diminished significantly the percent of dividing hAMMSCs contacted with leukemic Jurkat T cells. RTCA system showed significant hAMMSC invasion towards tumor cells and not vice versa. For all this, cellular interaction led to increasing viability of Jurkat T cells and decreasing hAMMSC viability. Thus, tumor Jurkat T cells could control a fate of health hAMMSCs and promote stromal microenvironment for survivability of tumor clones by means of secretable molecular products. Multilevel micro-arc CP coating forms bone-like inorganic structure that is capable to modulate in vitro interaction of human MMSCs and leukemic T lymphoblasts. The results obtained may be useful for replacement surgery applications of orthopedic implants in cancer patients.",
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