Biosynthesis of the scFv antibody to human ferritin in plant and bacterial producers

E. G. Semenyuk, O. A. Stremovskii, I. V. Orlova, T. G. Balandin, A. M. Nosov, Ya I. Buryanov, S. M. Deyev, R. V. Petrov

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and solubility. Production of the recombinant protein did not differ between parental transgenic plants and their first-generation progeny. Fusion with barstar allowed not only immunochemical detection of the recombinant scFv antibody, but also their purification from the plant material by affinity chromatography with barnase-His6 immobilized on a metal-affinity carrier.

Original languageEnglish
Pages (from-to)916-923
Number of pages8
JournalMolekulyarnaya Biologiya
Volume37
Issue number5
Publication statusPublished - 2003
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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