Biosynthesis of the scFv Antibody to Human Ferritin in Plant and Bacterial Producers

E. G. Semenyuk, O. A. Stremovskii, I. V. Orlova, T. G. Balandin, A. M. Nosov, Ya I. Buryanov, S. M. Deyev, R. V. Petrov

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and solubility. Production of the recombinant protein did not differ between parental transgenic plants and their first-generation progeny. Fusion with barstar allowed not only immunochemical detection of the recombinant scFv antibody, but also their purification from the plant material by affinity chromatography with barnase-His6 immobilized on a metal-affinity carrier.

Original languageEnglish
Pages (from-to)780-786
Number of pages7
JournalMolecular Biology
Volume37
Issue number5
DOIs
Publication statusPublished - Sep 2003
Externally publishedYes

Keywords

  • Barnase
  • Barstar
  • Transgenic plants
  • Variable antibody domains

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics

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    Semenyuk, E. G., Stremovskii, O. A., Orlova, I. V., Balandin, T. G., Nosov, A. M., Buryanov, Y. I., Deyev, S. M., & Petrov, R. V. (2003). Biosynthesis of the scFv Antibody to Human Ferritin in Plant and Bacterial Producers. Molecular Biology, 37(5), 780-786. https://doi.org/10.1023/A:1026005614970