[Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice].

M. A. Shevtsov, S. V. Shatik, [No Value] Tokarev, M. I. Mostova, L. A. Tiutin, N. V. Bychkova, A. L. Mikhrina, K. V. Onokhin, D. A. Meshalkina, I. V. Romanova, O. N. Savchenko, A. A. Ivanova, S. V. Abkin, V. A. Kharatian, I. V. Guzhova, B. A. Margulis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

For the first time, the biodistribution of recombinant heat shock protein in rhHsp70 rats with grafted intracranial C6 glioma was evaluated. It was assessed using the fluorescent antibody accumulation chaperone rhHsp70 conjugated with fluorochrome Alexa Fluor 555 in tumor cells by intratumoral or intravenous administration. Assessment of the distribution and accumulation of labeled protein was carried out on the model of subcutaneous B16/F10 melanoma in C57BL/6 mice with the use of single-photon emission computer tomography. After 60 minutes after intravenous administration rhHsp70-I123 (20 MBq, 5 mg chaperone) accumulation of the drug mainly in the liver and tumor tissue was showed. The coefficient of the differential accumulation of the labeled protein KDN(tumor/background) was 3.14. It was turned out that comparing the level of fixation of rhHsp70-I123 in the liver and the tumor KDN(tumor/ liver) = 0.76. After 24 hours from the time of injection of rhHsp70-I123 it was observed increase the level of fixation of the labeled protein in the liver and melanoma: KDN(tumor/background) = 3.43; KDN(tumor/liver = 0.78.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalVoprosy Onkologii
Volume59
Issue number2
Publication statusPublished - 2013

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Experimental Melanomas
Heat-Shock Proteins
Inbred C57BL Mouse
Recombinant Proteins
Glioma
Wistar Rats
Neoplasms
Liver
Intravenous Administration
Proteins
Fluorescent Dyes
Photons
Melanoma
Tomography
Injections
Antibodies
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Shevtsov, M. A., Shatik, S. V., Tokarev, N. V., Mostova, M. I., Tiutin, L. A., Bychkova, N. V., ... Margulis, B. A. (2013). [Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice]. Voprosy Onkologii, 59(2), 78-83.

[Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice]. / Shevtsov, M. A.; Shatik, S. V.; Tokarev, [No Value]; Mostova, M. I.; Tiutin, L. A.; Bychkova, N. V.; Mikhrina, A. L.; Onokhin, K. V.; Meshalkina, D. A.; Romanova, I. V.; Savchenko, O. N.; Ivanova, A. A.; Abkin, S. V.; Kharatian, V. A.; Guzhova, I. V.; Margulis, B. A.

In: Voprosy Onkologii, Vol. 59, No. 2, 2013, p. 78-83.

Research output: Contribution to journalArticle

Shevtsov, MA, Shatik, SV, Tokarev, NV, Mostova, MI, Tiutin, LA, Bychkova, NV, Mikhrina, AL, Onokhin, KV, Meshalkina, DA, Romanova, IV, Savchenko, ON, Ivanova, AA, Abkin, SV, Kharatian, VA, Guzhova, IV & Margulis, BA 2013, '[Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice].', Voprosy Onkologii, vol. 59, no. 2, pp. 78-83.
Shevtsov, M. A. ; Shatik, S. V. ; Tokarev, [No Value] ; Mostova, M. I. ; Tiutin, L. A. ; Bychkova, N. V. ; Mikhrina, A. L. ; Onokhin, K. V. ; Meshalkina, D. A. ; Romanova, I. V. ; Savchenko, O. N. ; Ivanova, A. A. ; Abkin, S. V. ; Kharatian, V. A. ; Guzhova, I. V. ; Margulis, B. A. / [Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice]. In: Voprosy Onkologii. 2013 ; Vol. 59, No. 2. pp. 78-83.
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abstract = "For the first time, the biodistribution of recombinant heat shock protein in rhHsp70 rats with grafted intracranial C6 glioma was evaluated. It was assessed using the fluorescent antibody accumulation chaperone rhHsp70 conjugated with fluorochrome Alexa Fluor 555 in tumor cells by intratumoral or intravenous administration. Assessment of the distribution and accumulation of labeled protein was carried out on the model of subcutaneous B16/F10 melanoma in C57BL/6 mice with the use of single-photon emission computer tomography. After 60 minutes after intravenous administration rhHsp70-I123 (20 MBq, 5 mg chaperone) accumulation of the drug mainly in the liver and tumor tissue was showed. The coefficient of the differential accumulation of the labeled protein KDN(tumor/background) was 3.14. It was turned out that comparing the level of fixation of rhHsp70-I123 in the liver and the tumor KDN(tumor/ liver) = 0.76. After 24 hours from the time of injection of rhHsp70-I123 it was observed increase the level of fixation of the labeled protein in the liver and melanoma: KDN(tumor/background) = 3.43; KDN(tumor/liver = 0.78.",
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AU - Shatik, S. V.

AU - Tokarev, [No Value]

AU - Mostova, M. I.

AU - Tiutin, L. A.

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AU - Meshalkina, D. A.

AU - Romanova, I. V.

AU - Savchenko, O. N.

AU - Ivanova, A. A.

AU - Abkin, S. V.

AU - Kharatian, V. A.

AU - Guzhova, I. V.

AU - Margulis, B. A.

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