[Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice].

M. A. Shevtsov, S. V. Shatik, [No Value] Tokarev, M. I. Mostova, L. A. Tiutin, N. V. Bychkova, A. L. Mikhrina, K. V. Onokhin, D. A. Meshalkina, I. V. Romanova, O. N. Savchenko, A. A. Ivanova, S. V. Abkin, V. A. Kharatian, I. V. Guzhova, B. A. Margulis

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Abstract

For the first time, the biodistribution of recombinant heat shock protein in rhHsp70 rats with grafted intracranial C6 glioma was evaluated. It was assessed using the fluorescent antibody accumulation chaperone rhHsp70 conjugated with fluorochrome Alexa Fluor 555 in tumor cells by intratumoral or intravenous administration. Assessment of the distribution and accumulation of labeled protein was carried out on the model of subcutaneous B16/F10 melanoma in C57BL/6 mice with the use of single-photon emission computer tomography. After 60 minutes after intravenous administration rhHsp70-I123 (20 MBq, 5 mg chaperone) accumulation of the drug mainly in the liver and tumor tissue was showed. The coefficient of the differential accumulation of the labeled protein KDN(tumor/background) was 3.14. It was turned out that comparing the level of fixation of rhHsp70-I123 in the liver and the tumor KDN(tumor/ liver) = 0.76. After 24 hours from the time of injection of rhHsp70-I123 it was observed increase the level of fixation of the labeled protein in the liver and melanoma: KDN(tumor/background) = 3.43; KDN(tumor/liver = 0.78.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalVoprosy Onkologii
Volume59
Issue number2
Publication statusPublished - 2013

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Shevtsov, M. A., Shatik, S. V., Tokarev, N. V., Mostova, M. I., Tiutin, L. A., Bychkova, N. V., Mikhrina, A. L., Onokhin, K. V., Meshalkina, D. A., Romanova, I. V., Savchenko, O. N., Ivanova, A. A., Abkin, S. V., Kharatian, V. A., Guzhova, I. V., & Margulis, B. A. (2013). [Biodistribution of the recombinant heat shock protein rhHsp70 in intracranial C6 glioma models in Wistar rats and subcutaneos B16/F10 melanoma in C57BL/6 mice]. Voprosy Onkologii, 59(2), 78-83.